FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567450320> ?p ?o ?g. }

• W1567450320 endingPage "4262" @default.
• W1567450320 startingPage "4259" @default.
• W1567450320 abstract "An engineered calmodulin differs from vertebrate calmodulin in its ability to activate Bordetella pertussis adenylate cyclase, and this difference has been utilized as the basis for a new purification protocol for the adenylate cyclase. VU-8 calmodulin, in which 3 glutamic acid residues (residues 82-84) have been substituted with 3 lysine residues, has a 1000-fold lower apparent affinity for the adenylate cyclase, compared to vertebrate calmodulin, and decreased maximal activity. Because of the relatively calcium-independent nature of the interaction between calmodulin and the cyclase, the use of calmodulin-Sepharose conjugates in the purification of the cyclase requires the use of chaotropic agents for elution. However, when immobilized VU-8 calmodulin was tested as a calcium-dependent, affinity-based, adsorption chromatography step in the purification of the cyclase from culture media or bacterial extracts, the enzyme bound to the column in a calcium-dependent manner, and a nearly homogeneous enzyme was obtained in high yield. These results demonstrate the feasibility of using engineered calmodulins that have selective differences in activity for the rational design of rapid purification protocols for calmodulin-binding proteins as well as indicate the importance of the conserved negative charge cluster at residues 82-84 of calmodulin for activation of this cyclase." @default.
• W1567450320 created "2016-06-24" @default.
• W1567450320 creator A5012799581 @default.
• W1567450320 creator A5029246358 @default.
• W1567450320 creator A5040126899 @default.
• W1567450320 creator A5045645139 @default.
• W1567450320 creator A5065266817 @default.
• W1567450320 creator A5077106047 @default.
• W1567450320 creator A5084301019 @default.
• W1567450320 date "1988-03-01" @default.
• W1567450320 modified "2023-09-28" @default.
• W1567450320 title "Affinity-based chromatography utilizing genetically engineered proteins. Interaction of Bordetella pertussis adenylate cyclase with calmodulin." @default.
• W1567450320 cites W1481302364 @default.
• W1567450320 cites W1517689470 @default.
• W1567450320 cites W1542129678 @default.
• W1567450320 cites W1568806088 @default.
• W1567450320 cites W1586520658 @default.
• W1567450320 cites W1598429267 @default.
• W1567450320 cites W1678098555 @default.
• W1567450320 cites W1775749144 @default.
• W1567450320 cites W1978952396 @default.
• W1567450320 cites W1995923973 @default.
• W1567450320 cites W1998493256 @default.
• W1567450320 cites W2051529934 @default.
• W1567450320 cites W2055252975 @default.
• W1567450320 cites W2058191493 @default.
• W1567450320 cites W2093171963 @default.
• W1567450320 cites W2100837269 @default.
• W1567450320 cites W2119460480 @default.
• W1567450320 cites W972369438 @default.
• W1567450320 doi "https://doi.org/10.1016/s0021-9258(18)68918-5" @default.
• W1567450320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2894377" @default.
• W1567450320 hasPublicationYear "1988" @default.
• W1567450320 type Work @default.
• W1567450320 sameAs 1567450320 @default.
• W1567450320 citedByCount "15" @default.
• W1567450320 countsByYear W15674503202014 @default.
• W1567450320 crossrefType "journal-article" @default.
• W1567450320 hasAuthorship W1567450320A5012799581 @default.
• W1567450320 hasAuthorship W1567450320A5029246358 @default.
• W1567450320 hasAuthorship W1567450320A5040126899 @default.
• W1567450320 hasAuthorship W1567450320A5045645139 @default.
• W1567450320 hasAuthorship W1567450320A5065266817 @default.
• W1567450320 hasAuthorship W1567450320A5077106047 @default.
• W1567450320 hasAuthorship W1567450320A5084301019 @default.
• W1567450320 hasBestOaLocation W15674503201 @default.
• W1567450320 hasConcept C104317684 @default.
• W1567450320 hasConcept C170493617 @default.
• W1567450320 hasConcept C179247698 @default.
• W1567450320 hasConcept C180526460 @default.
• W1567450320 hasConcept C181199279 @default.
• W1567450320 hasConcept C185592680 @default.
• W1567450320 hasConcept C2777582894 @default.
• W1567450320 hasConcept C2780069284 @default.
• W1567450320 hasConcept C2910325308 @default.
• W1567450320 hasConcept C29688787 @default.
• W1567450320 hasConcept C3019111730 @default.
• W1567450320 hasConcept C49773422 @default.
• W1567450320 hasConcept C51456166 @default.
• W1567450320 hasConcept C523546767 @default.
• W1567450320 hasConcept C54355233 @default.
• W1567450320 hasConcept C55493867 @default.
• W1567450320 hasConcept C80631254 @default.
• W1567450320 hasConcept C86803240 @default.
• W1567450320 hasConceptScore W1567450320C104317684 @default.
• W1567450320 hasConceptScore W1567450320C170493617 @default.
• W1567450320 hasConceptScore W1567450320C179247698 @default.
• W1567450320 hasConceptScore W1567450320C180526460 @default.
• W1567450320 hasConceptScore W1567450320C181199279 @default.
• W1567450320 hasConceptScore W1567450320C185592680 @default.
• W1567450320 hasConceptScore W1567450320C2777582894 @default.
• W1567450320 hasConceptScore W1567450320C2780069284 @default.
• W1567450320 hasConceptScore W1567450320C2910325308 @default.
• W1567450320 hasConceptScore W1567450320C29688787 @default.
• W1567450320 hasConceptScore W1567450320C3019111730 @default.
• W1567450320 hasConceptScore W1567450320C49773422 @default.
• W1567450320 hasConceptScore W1567450320C51456166 @default.
• W1567450320 hasConceptScore W1567450320C523546767 @default.
• W1567450320 hasConceptScore W1567450320C54355233 @default.
• W1567450320 hasConceptScore W1567450320C55493867 @default.
• W1567450320 hasConceptScore W1567450320C80631254 @default.
• W1567450320 hasConceptScore W1567450320C86803240 @default.
• W1567450320 hasIssue "9" @default.
• W1567450320 hasLocation W15674503201 @default.
• W1567450320 hasOpenAccess W1567450320 @default.
• W1567450320 hasPrimaryLocation W15674503201 @default.
• W1567450320 hasRelatedWork W1484283460 @default.
• W1567450320 hasRelatedWork W1509613015 @default.
• W1567450320 hasRelatedWork W1567450320 @default.
• W1567450320 hasRelatedWork W167497454 @default.
• W1567450320 hasRelatedWork W1971623716 @default.
• W1567450320 hasRelatedWork W1995923973 @default.
• W1567450320 hasRelatedWork W2045203765 @default.
• W1567450320 hasRelatedWork W2048895914 @default.
• W1567450320 hasRelatedWork W2274358699 @default.
• W1567450320 hasRelatedWork W2528831461 @default.
• W1567450320 hasVolume "263" @default.
• W1567450320 isParatext "false" @default.

• next