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- W1567451716 abstract "This chapter presents a study focusing on the measurement of glucose using simultaneous fluorescence quenching of two indicators. The utilization of tris (2,2'-bipyridyl) ruthenium(II) chloride hexahydrate (7 μM), and 1-hydroxypyrene-3,6,8-trisulfonic acid (HPTS) (0.03 μM), as fluorescent indicators, for the simultaneous detection of the enzymatic oxidation of glucose is discussed. The common excitation and fluorescence wavelengths for a solution containing both of the above indicators mixed together are 412 nm and 565 nm, respectively. The spectral properties of each indicator were studied separately, as well as together. The optimum operating conditions of the above systems with respect to concentrations of oxygen and hydrogen peroxide, and the pH of the system were established. There was no pH effect, a negligible effect of hydrogen peroxide concentration, and oxygen content had a significant effect on the spectral properties of tris(2,2'-bipyridyl) ruthenium(II) chloride hexahydrate. The spectra of HPTS were unaffected by different concentrations of oxygen and hydrogen peroxide. However, spectral properties were altered by changing the pH of the HPTS indicator solution. No interference was observed when using the combined mixture of both indicators." @default.
- W1567451716 created "2016-06-24" @default.
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- W1567451716 date "1994-01-01" @default.
- W1567451716 modified "2023-09-26" @default.
- W1567451716 title "The Measurement of Glucose using Simultaneous Fluorescence Quenching of Two Indicators" @default.
- W1567451716 doi "https://doi.org/10.1016/b978-1-85617-242-4.50192-0" @default.
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