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- W1567468713 abstract "8, 5'-Anhydro-8-oxyadenosine was phosphorylated using cyanoethylphosphate and dicyclohexylcarbodiimide (DCC) in dimethylformamide-pyridine. Treatment of the products with a mixture of 50% aqueous pyridine-triethylamine and DEAE-cellulose chromatography gave 2', 3'-cyclic phosphate in a yield of 48%. 8, 5'-Anhydro-8-oxyadenosine 3'-phosphate was obtained by RNase M digestion of the cyclic phosphate. After protection with benzoic anhydride in tetraethylammonium benzoate buffer, the 3'-phosphate was condensed with 2'-O-benzoyluridylyl-(3'→5')-N2, 2', 3'-O-triisobutyrylguanosine by the use of DCC. DEAE-cellulose, Sephadex G-25 and G-15 column chromatography gave 8, 5'-anhydro-8-oxyadenylyl-(3'→5')-uridylyl-(3'→5')-guanosine. The trinucleotide was characterized by degradation to cyclo Ap, Up and G with RNase M." @default.
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- W1567468713 date "1974-01-01" @default.
- W1567468713 modified "2023-09-27" @default.
- W1567468713 title "Polynucleotides. XX. Synthesis of an AUG Analog containing 8, 5'-O-Cycloadenosine" @default.
- W1567468713 doi "https://doi.org/10.1248/cpb.22.123" @default.
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