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• W1567507926 abstract "For establishment of a method for rapid diagnosis of Mycoplasma pneumoniae in clinical specimens, we designed and evaluated a loop-mediated isothermal amplification (LAMP) assay, using a set of primers targeting the SDC1 repetitive element of the M. pneumoniae genome.The method showed rapid and specific amplification for all the strains of M. pneumoniae tested (Type Iand II), within 1hour at 65°C.No cross-reactivity with the most common causative organisms bacterial pneumonia was observed.The detection limit was shown as 6 copies, which was equal to or higher than that of the two nested PCRs used as references.Two hundred four clinical samples, comprising sputum samples, throat swabs, etc., were tested by both LAMP and PCR, and determination of the correlations revealed complete agreement individually (24 posi-tives).The LAMP assay, a simple procedure in a closed system, allowed rapid amplification and accurate de-tection consistently; therefore we considered that it could become an caeasily available diagnostic methodsuitable for clinical situations requiring quick and appropriate decisions for treatments and care." @default.
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• W1567507926 date "2008-01-01" @default.
• W1567507926 modified "2023-09-28" @default.
• W1567507926 title "Sensitive and Rapid Detection of Mycoplasma pneumoniae by Loop-mediated Isothermal Amplification" @default.
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• W1567507926 doi "https://doi.org/10.11150/kansenshogakuzasshi1970.82.168" @default.
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