FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567642163> ?p ?o ?g. }

• W1567642163 endingPage "357" @default.
• W1567642163 startingPage "348" @default.
• W1567642163 abstract "Cationic cell wall‐bound peroxidase (CWPO‐C) has the capability to oxidize sinapyl alcohol, ferrocytochrome c , and synthetic lignin polymers, unlike most peroxidases that have been characterized in flowering plants, such as horseradish peroxidase and Arabidopsis thaliana peroxidase A2. It has been suggested that the oxidation site is located on the CWPO‐C surface, and homology modeling and chemically modified CWPO‐C studies suggest that Tyr74 and/or Tyr177 are possible participants in the catalytic site. The present study clarifies the importance of these Tyr residues for substrate oxidation, using recombinant CWPO‐C and recombinant mutant CWPO‐C with phenylalanine substitution(s) for tyrosine. Such recombinant proteins, produced in Escherichia coli as inclusion bodies, were successfully refolded to yield the active form, and purified recombinant protein solutions exhibited typical spectra of high‐spin ferric protein and displayed H 2 O 2 ‐dependent oxidation of guaiacol, 2,6‐dimethoxyphenol, and syringaldazine. Measurement of peroxidase activity with these guaiacyl and syringyl compounds as reducing substrates indicated that a single mutation, Y74F or Y177F, resulted in substantial loss of oxidation activity (∼ 40–60% and 82%, respectively). Also, over 95% of the oxidation activity was lost with a double mutation, Y74F/Y177F. These results indicated that Tyr74 and Tyr177, rather than the heme pocket, play a central role in the oxidation of these substrates. This is the first report of active residues on an enzyme surface being identified in a plant peroxidase. This study also suggests that sinapyl alcohol incorporation into lignin is performed by a peroxidase that generates Tyr radicals on its surface." @default.
• W1567642163 created "2016-06-24" @default.
• W1567642163 creator A5035207671 @default.
• W1567642163 creator A5054935609 @default.
• W1567642163 creator A5056392157 @default.
• W1567642163 creator A5088210319 @default.
• W1567642163 date "2011-12-09" @default.
• W1567642163 modified "2023-10-18" @default.
• W1567642163 title "Identification of Tyr74 and Tyr177 as substrate oxidation sites in cationic cell wall-bound peroxidase from Populus alba L." @default.
• W1567642163 cites W1486937943 @default.
• W1567642163 cites W1490343139 @default.
• W1567642163 cites W1502369780 @default.
• W1567642163 cites W1534246196 @default.
• W1567642163 cites W1606032544 @default.
• W1567642163 cites W1968738464 @default.
• W1567642163 cites W1975005310 @default.
• W1567642163 cites W1977071852 @default.
• W1567642163 cites W1980073565 @default.
• W1567642163 cites W1981673656 @default.
• W1567642163 cites W1982083268 @default.
• W1567642163 cites W1985236672 @default.
• W1567642163 cites W1990567116 @default.
• W1567642163 cites W1991336972 @default.
• W1567642163 cites W1993391297 @default.
• W1567642163 cites W1995430405 @default.
• W1567642163 cites W1996138707 @default.
• W1567642163 cites W2012684695 @default.
• W1567642163 cites W2016830482 @default.
• W1567642163 cites W2018524395 @default.
• W1567642163 cites W2019390366 @default.
• W1567642163 cites W2022978286 @default.
• W1567642163 cites W2031330779 @default.
• W1567642163 cites W2032839609 @default.
• W1567642163 cites W2039240730 @default.
• W1567642163 cites W2039729590 @default.
• W1567642163 cites W2042770659 @default.
• W1567642163 cites W2045106068 @default.
• W1567642163 cites W2049642961 @default.
• W1567642163 cites W2054216679 @default.
• W1567642163 cites W2072679497 @default.
• W1567642163 cites W2085501958 @default.
• W1567642163 cites W2087790671 @default.
• W1567642163 cites W2088827101 @default.
• W1567642163 cites W2093033663 @default.
• W1567642163 cites W2093067443 @default.
• W1567642163 cites W2094798179 @default.
• W1567642163 cites W2102159048 @default.
• W1567642163 cites W2113977229 @default.
• W1567642163 cites W2115302073 @default.
• W1567642163 cites W2127870464 @default.
• W1567642163 cites W2131560052 @default.
• W1567642163 cites W2137839040 @default.
• W1567642163 cites W2152301430 @default.
• W1567642163 cites W2161021879 @default.
• W1567642163 cites W2161197293 @default.
• W1567642163 cites W2171655733 @default.
• W1567642163 cites W28056765 @default.
• W1567642163 doi "https://doi.org/10.1111/j.1742-4658.2011.08429.x" @default.
• W1567642163 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22099451" @default.
• W1567642163 hasPublicationYear "2011" @default.
• W1567642163 type Work @default.
• W1567642163 sameAs 1567642163 @default.
• W1567642163 citedByCount "19" @default.
• W1567642163 countsByYear W15676421632013 @default.
• W1567642163 countsByYear W15676421632014 @default.
• W1567642163 countsByYear W15676421632015 @default.
• W1567642163 countsByYear W15676421632016 @default.
• W1567642163 countsByYear W15676421632017 @default.
• W1567642163 countsByYear W15676421632018 @default.
• W1567642163 countsByYear W15676421632019 @default.
• W1567642163 countsByYear W15676421632020 @default.
• W1567642163 countsByYear W15676421632022 @default.
• W1567642163 countsByYear W15676421632023 @default.
• W1567642163 crossrefType "journal-article" @default.
• W1567642163 hasAuthorship W1567642163A5035207671 @default.
• W1567642163 hasAuthorship W1567642163A5054935609 @default.
• W1567642163 hasAuthorship W1567642163A5056392157 @default.
• W1567642163 hasAuthorship W1567642163A5088210319 @default.
• W1567642163 hasConcept C162008176 @default.
• W1567642163 hasConcept C178790620 @default.
• W1567642163 hasConcept C181199279 @default.
• W1567642163 hasConcept C185592680 @default.
• W1567642163 hasConcept C2780199054 @default.
• W1567642163 hasConcept C2781052789 @default.
• W1567642163 hasConcept C41183919 @default.
• W1567642163 hasConcept C55493867 @default.
• W1567642163 hasConcept C71240020 @default.
• W1567642163 hasConceptScore W1567642163C162008176 @default.
• W1567642163 hasConceptScore W1567642163C178790620 @default.
• W1567642163 hasConceptScore W1567642163C181199279 @default.
• W1567642163 hasConceptScore W1567642163C185592680 @default.
• W1567642163 hasConceptScore W1567642163C2780199054 @default.
• W1567642163 hasConceptScore W1567642163C2781052789 @default.
• W1567642163 hasConceptScore W1567642163C41183919 @default.
• W1567642163 hasConceptScore W1567642163C55493867 @default.
• W1567642163 hasConceptScore W1567642163C71240020 @default.
• W1567642163 hasIssue "2" @default.
• W1567642163 hasLocation W15676421631 @default.

• next