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- W1567677182 abstract "Chick genomic DNA containing the extreme 5' end of the alpha 2 (type I) collagen gene has been used as template in an in vitro HeLa cell transcription system. RNA polymerase II-dependent transcription initiates from a specific site on this DNA. The precise location of this site was determined by three types of experiments: sizing of in vitro-synthesized RNA runoff transcripts, comparing the sequence of the in vitro-made RNA transcripts with the structure of the DNA template, and identifying the first and second nucleotides of the in vitro-synthesized transcripts. Transcription was found to initiate 33 base pairs downstream from a canonical Goldberg-Hogness sequence (TATAAATA). This in vitro start site is the same as the initiation site of in vivo-synthesized collagen RNA." @default.
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- W1567677182 date "1981-11-01" @default.
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- W1567677182 title "Accurate in vitro transcriptional initiation of the chick alpha 2 (Type I) collagen gene." @default.
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- W1567677182 doi "https://doi.org/10.1016/s0021-9258(19)68585-6" @default.
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