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• W1567678719 abstract "This unit describes protocols used in the production of chimeric mice that are then used for the generation of gene knockout mice. These protocols include the collection of blastocyst embryos, ES cell injection, and uterine transfer of injected blastocysts. Support protocols for the superovulation of blastocyst donor mice, generation of pseudopregnant recipients, fabrication of glass pipets for embryo and cell manipulations, and generation of germline mice are also included. Practical tips and solutions are mentioned to help troubleshoot problems that may occur." @default.
• W1567678719 created "2016-06-24" @default.
• W1567678719 creator A5031655398 @default.
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• W1567678719 date "2009-09-01" @default.
• W1567678719 modified "2023-10-18" @default.
• W1567678719 title "Generation of Gene Knockout Mice by ES Cell Microinjection" @default.
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• W1567678719 doi "https://doi.org/10.1002/0471143030.cb1914s44" @default.
• W1567678719 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2759098" @default.
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