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- W1567682040 abstract "Abstract Nonlethal DNA sampling is a highly recommendable method in molecular genetic studies of protected and endangered species. To develop a demonstrably nonlethal method of obtaining DNA from larvae of endangered diving beetles ( C ybister brevis , C . lewisianus , C . limbatus , C . rugosus , D ytiscus sharpi sharpi and D . sharpi validus ), we obtained the larval exuvia (molted skin) of these endangered diving beetles under laboratory conditions. A single exuvia 24 h after molting was sufficient to allow polymerase chain reaction ( PCR ) detection of a mitochondrial DNA gene, cytochrome‐c oxidase subunit I ( COI ), and the sequence of the COI gene could be determined directly. Sequences obtained from the exuvial samples were used to further find similarities within DDBJ / EMBL / G en B ank. Genomic DNA from the samples was successfully isolated, and we identified the species. This process suggests that exuvia provides a good sample for extracting DNA from endangered diving beetle larvae without killing them." @default.
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- W1567682040 date "2015-04-07" @default.
- W1567682040 modified "2023-10-05" @default.
- W1567682040 title "Noninvasive sampling of DNA from larval exuvia in diving beetles of the genera<i>Cybister</i>and<i>Dytiscus</i>" @default.
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- W1567682040 doi "https://doi.org/10.1111/ens.12129" @default.
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