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- W1567747098 abstract "The correction of human genetic disorders by transfer of genetic materialto cells is under intensive investigation in a number of 1aboratories.One possible way of trying to achieve the transfer of nucleic acid is byattaching DNA to a protein which has specific receptors on cells and whichundergoes receptor-mediated endocytosis.In order to make use of the ligand protein-receptor approach for DNA transfer,iron-loaded human serum transferrin has been modified with the water solublecarbodiimides N-ethy1-N I -(3-dilllethy1aminopropyl) carbodiimide (CDI) andits quaterary analogue (ECDI) to give modified N-acy1urea transferrins.N-Acy1urea CDI (Fe 3+) transferrin and N-acy1urea CDI (Fe ) transferrinhave been found to interact with and bind DNA in a reversible manner whichi! dependent on ionic strength.[1251] N-Acy1urea CDI+(Fe3+) transferrin binds to transferrin receptorson Hea cells in culture and undergoes internalization through receptor-mediatedendocytosis. Binding of the modified transferrin in the presenceof calf thymus DNA to transferrin receptors also takes place. However, althoughinternalization in the presence of DNA doe! appear to take place, theresults of the internalization are not fully understood.Transfection studies with N-acy1urea CDI (Fe ) transferrin and plasmidpBR322 DNA as well as plasmid ptkNEO DNA complexes in the HeLa cell systemare reported. The results of a number of transfection experiments suggeststhat N-acy1urea transferrins are capable of transfecting DNA (ptkNEO DNA),carrying genes for resistance to the antibiotic Geneticin (G41S) in theHeLa cell system. However, further development of the transfection systemis necessary in order that consistantly reproducible results may be achievd." @default.
- W1567747098 created "2016-06-24" @default.
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- W1567747098 date "1986-01-01" @default.
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- W1567747098 title "Studies on the preparation and interaction of modified transferrin-DNA complexes with HeLa cells." @default.
- W1567747098 hasPublicationYear "1986" @default.
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