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- W1567755710 abstract "Radiolabeled donor DNA is efficiently taken up into competent H. influenzae Rd rec-2 mutant cells but does not undergo the rapid degradation observed in wild-type cells. Furthermore, donor label is not recovered in the chromosome even after 1 h. The donor DNA appears to remain in a protected state in a compartment that can be separated from the rest of the cell. We interpret this as a failure of the donor DNA to be translocated out of the transformasome. In contrast, rec-1 cells translocate labeled donor DNA normally. The donor label accumulates in the recipient chromosome, but, as expected for cells with a recombination defect, there is no preferential localization of the label in sites homologous to the donor DNA. In addition, we have observed two enzymatic activities that act on transformasome-associated DNA of rec-2 cells, an endonuclease which may play a role in the translocation of closed circular DNA and a phosphatase." @default.
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- W1567755710 date "1985-08-01" @default.
- W1567755710 modified "2023-10-15" @default.
- W1567755710 title "Reexamination of phenotypic defects in rec-1 and rec-2 mutants of Haemophilus influenzae Rd" @default.
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- W1567755710 doi "https://doi.org/10.1128/jb.163.2.629-634.1985" @default.
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