FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567756114> ?p ?o ?g. }

• W1567756114 endingPage "6677" @default.
• W1567756114 startingPage "6670" @default.
• W1567756114 abstract "Since eucaryotic cell-derived thymidine or thymidine nucleotides are not incorporated into Chlamydia trachomatis DNA, we hypothesized that C. trachomatis must obtain dTTP for DNA synthesis by converting dUMP to dTMP. In most cells, this reaction is catalyzed by thymidylate synthase (TS) and requires 5,10-methylenetetrahydrofolate as a cofactor. We used C. trachomatis serovar L2 and a mutant CHO K1 cell line with a genetic deficiency in folate metabolism as a host for chlamydial growth. This cell line lacks a functional dihydrofolate reductase (DHFR) gene and, as a result, is unable to carry out de novo synthesis of dTTP. C. trachomatis inclusions form normally when DHFR- cells are starved for thymidine 24 h prior to and during the course of infection. When [6-3H]uridine is used as a precursor to label C. trachomatis-infected CHO DHFR- cells, radiolabel is readily incorporated into chlamydia-specific DNA. When DNA from [6-3H]uridine-labelled infected cultures is acid hydrolyzed and subjected to high-performance liquid chromatography analysis, radiolabel is detected in thymine and cytosine nucleobases. By using the DHFR- cell line as a host and [5-3H]uridine as a precursor, we could monitor intracellular C. trachomatis TS activity simply by following the formation of tritiated water. There is a good correlation between in situ TS activity and DNA synthesis activity during the chlamydial growth cycle. In addition, both C. trachomatis-specific DNA synthesis and 3H2O release are inhibited by exogenously added 5-fluorouridine but not by 5-fluorodeoxyuridine. Finally, we demonstrated in vitro TS activity in crude extracts prepared from highly purified C. trachomatis reticulate bodies. The activity is dependent on the presence of methylenetetrahydrofolic acid and can be inhibited with 5-fluoro-dUMP. Taken together, these results indicate that C. trachomatis contains a TS for the synthesis of dTMP." @default.
• W1567756114 created "2016-06-24" @default.
• W1567756114 creator A5032047953 @default.
• W1567756114 creator A5047587564 @default.
• W1567756114 creator A5089610748 @default.
• W1567756114 date "1991-11-01" @default.
• W1567756114 modified "2023-09-23" @default.
• W1567756114 title "Biochemical evidence for the existence of thymidylate synthase in the obligate intracellular parasite Chlamydia trachomatis" @default.
• W1567756114 cites W1509061163 @default.
• W1567756114 cites W1512473402 @default.
• W1567756114 cites W1539539213 @default.
• W1567756114 cites W1562920995 @default.
• W1567756114 cites W157990370 @default.
• W1567756114 cites W1602254003 @default.
• W1567756114 cites W1615168074 @default.
• W1567756114 cites W1668637796 @default.
• W1567756114 cites W1890701869 @default.
• W1567756114 cites W1937435647 @default.
• W1567756114 cites W1963495259 @default.
• W1567756114 cites W1967830103 @default.
• W1567756114 cites W1973319885 @default.
• W1567756114 cites W1974781113 @default.
• W1567756114 cites W1976492411 @default.
• W1567756114 cites W1977882075 @default.
• W1567756114 cites W2014062936 @default.
• W1567756114 cites W2017566107 @default.
• W1567756114 cites W2047549095 @default.
• W1567756114 cites W2057045284 @default.
• W1567756114 cites W2070345146 @default.
• W1567756114 cites W2093657716 @default.
• W1567756114 cites W2096725836 @default.
• W1567756114 cites W2129905248 @default.
• W1567756114 cites W2144163230 @default.
• W1567756114 cites W2167847781 @default.
• W1567756114 cites W2327719826 @default.
• W1567756114 cites W4211183610 @default.
• W1567756114 cites W4229857979 @default.
• W1567756114 cites W4235480170 @default.
• W1567756114 cites W71090974 @default.
• W1567756114 doi "https://doi.org/10.1128/jb.173.21.6670-6677.1991" @default.
• W1567756114 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/209014" @default.
• W1567756114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1938873" @default.
• W1567756114 hasPublicationYear "1991" @default.
• W1567756114 type Work @default.
• W1567756114 sameAs 1567756114 @default.
• W1567756114 citedByCount "16" @default.
• W1567756114 countsByYear W15677561142014 @default.
• W1567756114 crossrefType "journal-article" @default.
• W1567756114 hasAuthorship W1567756114A5032047953 @default.
• W1567756114 hasAuthorship W1567756114A5047587564 @default.
• W1567756114 hasAuthorship W1567756114A5089610748 @default.
• W1567756114 hasBestOaLocation W15677561142 @default.
• W1567756114 hasConcept C104317684 @default.
• W1567756114 hasConcept C123321153 @default.
• W1567756114 hasConcept C153911025 @default.
• W1567756114 hasConcept C159047783 @default.
• W1567756114 hasConcept C181199279 @default.
• W1567756114 hasConcept C2776694085 @default.
• W1567756114 hasConcept C2776858809 @default.
• W1567756114 hasConcept C2777108182 @default.
• W1567756114 hasConcept C2777391075 @default.
• W1567756114 hasConcept C2780456651 @default.
• W1567756114 hasConcept C2781259782 @default.
• W1567756114 hasConcept C2781320022 @default.
• W1567756114 hasConcept C54355233 @default.
• W1567756114 hasConcept C552990157 @default.
• W1567756114 hasConcept C55493867 @default.
• W1567756114 hasConcept C64241487 @default.
• W1567756114 hasConcept C67705224 @default.
• W1567756114 hasConcept C86803240 @default.
• W1567756114 hasConceptScore W1567756114C104317684 @default.
• W1567756114 hasConceptScore W1567756114C123321153 @default.
• W1567756114 hasConceptScore W1567756114C153911025 @default.
• W1567756114 hasConceptScore W1567756114C159047783 @default.
• W1567756114 hasConceptScore W1567756114C181199279 @default.
• W1567756114 hasConceptScore W1567756114C2776694085 @default.
• W1567756114 hasConceptScore W1567756114C2776858809 @default.
• W1567756114 hasConceptScore W1567756114C2777108182 @default.
• W1567756114 hasConceptScore W1567756114C2777391075 @default.
• W1567756114 hasConceptScore W1567756114C2780456651 @default.
• W1567756114 hasConceptScore W1567756114C2781259782 @default.
• W1567756114 hasConceptScore W1567756114C2781320022 @default.
• W1567756114 hasConceptScore W1567756114C54355233 @default.
• W1567756114 hasConceptScore W1567756114C552990157 @default.
• W1567756114 hasConceptScore W1567756114C55493867 @default.
• W1567756114 hasConceptScore W1567756114C64241487 @default.
• W1567756114 hasConceptScore W1567756114C67705224 @default.
• W1567756114 hasConceptScore W1567756114C86803240 @default.
• W1567756114 hasIssue "21" @default.
• W1567756114 hasLocation W15677561141 @default.
• W1567756114 hasLocation W15677561142 @default.
• W1567756114 hasLocation W15677561143 @default.
• W1567756114 hasLocation W15677561144 @default.
• W1567756114 hasOpenAccess W1567756114 @default.
• W1567756114 hasPrimaryLocation W15677561141 @default.
• W1567756114 hasRelatedWork W1213032464 @default.
• W1567756114 hasRelatedWork W1542780738 @default.
• W1567756114 hasRelatedWork W1567756114 @default.

• next