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- W1567778831 endingPage "714" @default.
- W1567778831 startingPage "708" @default.
- W1567778831 abstract "Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. Transformation analyses identified a specific mutation, designated ngr-374, that was responsible not only for N-methyl-N'-nitro-N-nitrosoguanidine sensitivity, but also sensitivity to methyl methanesulfonate, ethyl methanesulfonate, nitrous acid, and UV irradiation. However, ngr-374-carrying recombinants showed no significant increase in their sensitivity to mitomycin C or 4-nitroquinoline 1-oxide and were unaffected in recombination proficiency. In vitro assays showed that ngr-374-carrying strains had lower apurinic/apyrimidinic endonuclease activities than the wild type. The chromosomal locus occupied by ngr-374 was shown to exist in the gene order omega(Chr::Tn551)40-ngr-374-thrB106." @default.
- W1567778831 created "2016-06-24" @default.
- W1567778831 creator A5032810952 @default.
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- W1567778831 date "1986-11-01" @default.
- W1567778831 modified "2023-09-26" @default.
- W1567778831 title "Characterization and genetic mapping of a mutation affecting apurinic endonuclease activity in Staphylococcus aureus" @default.
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- W1567778831 doi "https://doi.org/10.1128/jb.168.2.708-714.1986" @default.
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