FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567800226> ?p ?o ?g. }

• W1567800226 endingPage "404" @default.
• W1567800226 startingPage "393" @default.
• W1567800226 abstract "An acidic Ca2+-independent phospholipase A2 (aiPLA2) has been isolated previously from rat lung and a human cDNA has been described. This study applied the method to larger scale isolation of the native protein from the bovine lung. A polyclonal antibody was generated to a 15 amino acid synthetic peptide based on a conserved rat/human sequence. This antibody recognized a single protein band with an estimated molecular mass of ≈29 kDa in a soluble fraction obtained from bovine lung homogenate. A 29 kDa protein that reacted with the aiPLA2 antipeptide antibody was detected in fractions containing aiPLA2 activity on sequential column chromatographies. The partially purified enzyme showed 176-fold increase over the homogenate in Ca2+-independent PLA2 activity at pH 4. Activity was maximal with phosphatidylcholine substrate and was significantly less with phosphatidylethanolamine and anionic phospholipids. The enzyme had no acyl group preference in phosphatidylcholine and showed no preference for oxidized substrate, but activity was less with 1-O-alkyl phosphatidylcholine. aiPLA2 activity was inhibited by a transition state phospholipid analog (MJ33, 1-hexadecyl-3-trifluoroethylglycero-sn-2-phosphomethanol), serine protease inhibitors, and the anti-peptide antibody but was insensitive to arachidonoyl trifluoromethyl ketone, bromoenol lactone, p-bromophenacyl bromide, and ATP. Analysis of N-terminal amino acid sequence for the 29 kDa protein demonstrated its high homology to human 26 kDa aiPLA2. These was no significant change in molecular mass of the protein following treatment with endoglycosidase F. Western blot of subcellular fractions from rat lung indicated aiPLA2 immunoreactivity with lamellar body, lysosomal, and cytosolic fractions. These results indicate isolation from bovine lung of a 29 kDa acidic Ca2+-independent phospholipase A2 homologue of the rat and human enzyme and provide evidence for specificity in the metabolism of lung surfactant phosphatidylcholine." @default.
• W1567800226 created "2016-06-24" @default.
• W1567800226 creator A5008171524 @default.
• W1567800226 creator A5021560480 @default.
• W1567800226 creator A5022854559 @default.
• W1567800226 creator A5062453760 @default.
• W1567800226 date "1998-06-01" @default.
• W1567800226 modified "2023-09-27" @default.
• W1567800226 title "Characterization of acidic Ca2+-independent phospholipase A2 of bovine lung" @default.
• W1567800226 cites W1512762418 @default.
• W1567800226 cites W1516262714 @default.
• W1567800226 cites W1562005093 @default.
• W1567800226 cites W1567170846 @default.
• W1567800226 cites W1573258040 @default.
• W1567800226 cites W1577662565 @default.
• W1567800226 cites W1801885837 @default.
• W1567800226 cites W1912590178 @default.
• W1567800226 cites W1987614973 @default.
• W1567800226 cites W1993378893 @default.
• W1567800226 cites W1994915412 @default.
• W1567800226 cites W1998433873 @default.
• W1567800226 cites W2003222248 @default.
• W1567800226 cites W2008895292 @default.
• W1567800226 cites W2011661565 @default.
• W1567800226 cites W2012397093 @default.
• W1567800226 cites W2017384797 @default.
• W1567800226 cites W2059727135 @default.
• W1567800226 cites W2069404833 @default.
• W1567800226 cites W2074298401 @default.
• W1567800226 cites W2077300104 @default.
• W1567800226 cites W2080664733 @default.
• W1567800226 cites W2091783910 @default.
• W1567800226 cites W2093272291 @default.
• W1567800226 cites W2094404867 @default.
• W1567800226 cites W2109214472 @default.
• W1567800226 cites W2145187316 @default.
• W1567800226 cites W2168082981 @default.
• W1567800226 cites W2182242759 @default.
• W1567800226 cites W2209189960 @default.
• W1567800226 cites W2246467808 @default.
• W1567800226 cites W2310348403 @default.
• W1567800226 cites W2413073234 @default.
• W1567800226 cites W2418737946 @default.
• W1567800226 cites W4210963323 @default.
• W1567800226 cites W4292517095 @default.
• W1567800226 doi "https://doi.org/10.1016/s0305-0491(98)10046-9" @default.
• W1567800226 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9787801" @default.
• W1567800226 hasPublicationYear "1998" @default.
• W1567800226 type Work @default.
• W1567800226 sameAs 1567800226 @default.
• W1567800226 citedByCount "56" @default.
• W1567800226 countsByYear W15678002262012 @default.
• W1567800226 countsByYear W15678002262014 @default.
• W1567800226 countsByYear W15678002262015 @default.
• W1567800226 countsByYear W15678002262016 @default.
• W1567800226 countsByYear W15678002262017 @default.
• W1567800226 countsByYear W15678002262018 @default.
• W1567800226 countsByYear W15678002262019 @default.
• W1567800226 countsByYear W15678002262020 @default.
• W1567800226 countsByYear W15678002262022 @default.
• W1567800226 countsByYear W15678002262023 @default.
• W1567800226 crossrefType "journal-article" @default.
• W1567800226 hasAuthorship W1567800226A5008171524 @default.
• W1567800226 hasAuthorship W1567800226A5021560480 @default.
• W1567800226 hasAuthorship W1567800226A5022854559 @default.
• W1567800226 hasAuthorship W1567800226A5062453760 @default.
• W1567800226 hasBestOaLocation W15678002261 @default.
• W1567800226 hasConcept C104317684 @default.
• W1567800226 hasConcept C153911025 @default.
• W1567800226 hasConcept C167625842 @default.
• W1567800226 hasConcept C181199279 @default.
• W1567800226 hasConcept C185592680 @default.
• W1567800226 hasConcept C2776330855 @default.
• W1567800226 hasConcept C2778918659 @default.
• W1567800226 hasConcept C41625074 @default.
• W1567800226 hasConcept C55493867 @default.
• W1567800226 hasConcept C58121356 @default.
• W1567800226 hasConcept C86803240 @default.
• W1567800226 hasConceptScore W1567800226C104317684 @default.
• W1567800226 hasConceptScore W1567800226C153911025 @default.
• W1567800226 hasConceptScore W1567800226C167625842 @default.
• W1567800226 hasConceptScore W1567800226C181199279 @default.
• W1567800226 hasConceptScore W1567800226C185592680 @default.
• W1567800226 hasConceptScore W1567800226C2776330855 @default.
• W1567800226 hasConceptScore W1567800226C2778918659 @default.
• W1567800226 hasConceptScore W1567800226C41625074 @default.
• W1567800226 hasConceptScore W1567800226C55493867 @default.
• W1567800226 hasConceptScore W1567800226C58121356 @default.
• W1567800226 hasConceptScore W1567800226C86803240 @default.
• W1567800226 hasIssue "2" @default.
• W1567800226 hasLocation W15678002261 @default.
• W1567800226 hasLocation W15678002262 @default.
• W1567800226 hasOpenAccess W1567800226 @default.
• W1567800226 hasPrimaryLocation W15678002261 @default.
• W1567800226 hasRelatedWork W1967726508 @default.
• W1567800226 hasRelatedWork W1969999236 @default.
• W1567800226 hasRelatedWork W1982477616 @default.
• W1567800226 hasRelatedWork W2029617039 @default.

• next