FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567873484> ?p ?o ?g. }

• W1567873484 abstract "F9 teratocarcinoma is a useful model for studying early embryogenesis since these cells can differentiate into primitive or parietal endoderm under the influence of retinoic acid or retinoic acid and cyclic AMP, respectively. We have found that three isoforms of protein kinase C (PKC alpha, -beta, and -gamma) were expressed in undifferentiated stem cells. When the cells were treated with retinoic acid either alone or in the presence of cAMP for 120 h, PKC alpha mRNA and protein levels increased, whereas those of PKC beta and PKC gamma became undetectable. These changes began within 24 h of drug treatment and were complete by 48-72 h. In order to determine the functional significance of the induction of PKC alpha during F9 differentiation, we established two stable transfectants that overexpressed PKC alpha protein between 4- and 5-fold compared to wild type cells. Characterization of these cell lines revealed an altered pattern of expression of some of the markers of F9 differentiation. The clone that had the highest amount of PKC alpha protein constitutively expressed mRNA for type IV collagen and c-Jun, which are not normally expressed until 24-48 h of treatment with differentiation agents. In the other overexpressing clone, these markers were induced much faster than in wild type cells. The growth rate of both overexpressing clones was less than wild type cells, while the expression of the PKC beta protein in these clones was similar to the levels found in differentiated F9 cells. However, other markers of differentiation, including the cellular morphology and levels of pST6-135 and c-myc RNA, responded to agents identically in both wild type and PKC-alpha-overexpressing clones. Therefore, overexpression of PKC alpha is not sufficient to induce full differentiation of F9 cells. However, our data suggest that certain pathways that lead to the expression of differentiation-dependent genes are regulated by PKC alpha protein levels." @default.
• W1567873484 created "2016-06-24" @default.
• W1567873484 creator A5012809725 @default.
• W1567873484 creator A5020276288 @default.
• W1567873484 creator A5033835530 @default.
• W1567873484 creator A5067673954 @default.
• W1567873484 date "1994-11-01" @default.
• W1567873484 modified "2023-09-27" @default.
• W1567873484 title "Characterization of conventional protein kinase C (PKC) isotype expression during F9 teratocarcinoma differentiation. Overexpression of PKC alpha alters the expression of some differentiation-dependent genes." @default.
• W1567873484 cites W1517412176 @default.
• W1567873484 cites W1539267410 @default.
• W1567873484 cites W1570470675 @default.
• W1567873484 cites W1578152976 @default.
• W1567873484 cites W1773519006 @default.
• W1567873484 cites W1982961932 @default.
• W1567873484 cites W1986767025 @default.
• W1567873484 cites W1987897953 @default.
• W1567873484 cites W1992251993 @default.
• W1567873484 cites W1995499596 @default.
• W1567873484 cites W1997261857 @default.
• W1567873484 cites W2002468039 @default.
• W1567873484 cites W2005660376 @default.
• W1567873484 cites W2007319015 @default.
• W1567873484 cites W2009856702 @default.
• W1567873484 cites W2009886925 @default.
• W1567873484 cites W2025907609 @default.
• W1567873484 cites W2026819360 @default.
• W1567873484 cites W2032944908 @default.
• W1567873484 cites W2034994122 @default.
• W1567873484 cites W2037753946 @default.
• W1567873484 cites W2044133276 @default.
• W1567873484 cites W2045798063 @default.
• W1567873484 cites W2058744360 @default.
• W1567873484 cites W2066160869 @default.
• W1567873484 cites W2069584591 @default.
• W1567873484 cites W2070547356 @default.
• W1567873484 cites W2072392608 @default.
• W1567873484 cites W2073386386 @default.
• W1567873484 cites W2073548049 @default.
• W1567873484 cites W2086524338 @default.
• W1567873484 cites W2096077209 @default.
• W1567873484 cites W2103719933 @default.
• W1567873484 cites W2156172618 @default.
• W1567873484 cites W2158343596 @default.
• W1567873484 cites W2426793753 @default.
• W1567873484 cites W3127324270 @default.
• W1567873484 cites W4238441375 @default.
• W1567873484 cites W4249862524 @default.
• W1567873484 cites W4255858411 @default.
• W1567873484 cites W4294216491 @default.
• W1567873484 doi "https://doi.org/10.1016/s0021-9258(18)47050-0" @default.
• W1567873484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7961696" @default.
• W1567873484 hasPublicationYear "1994" @default.
• W1567873484 type Work @default.
• W1567873484 sameAs 1567873484 @default.
• W1567873484 citedByCount "35" @default.
• W1567873484 countsByYear W15678734842012 @default.
• W1567873484 countsByYear W15678734842015 @default.
• W1567873484 crossrefType "journal-article" @default.
• W1567873484 hasAuthorship W1567873484A5012809725 @default.
• W1567873484 hasAuthorship W1567873484A5020276288 @default.
• W1567873484 hasAuthorship W1567873484A5033835530 @default.
• W1567873484 hasAuthorship W1567873484A5067673954 @default.
• W1567873484 hasBestOaLocation W15678734841 @default.
• W1567873484 hasConcept C104317684 @default.
• W1567873484 hasConcept C115302286 @default.
• W1567873484 hasConcept C148738053 @default.
• W1567873484 hasConcept C153911025 @default.
• W1567873484 hasConcept C159110408 @default.
• W1567873484 hasConcept C184235292 @default.
• W1567873484 hasConcept C195794163 @default.
• W1567873484 hasConcept C2775944032 @default.
• W1567873484 hasConcept C2779963572 @default.
• W1567873484 hasConcept C2781121885 @default.
• W1567873484 hasConcept C49453240 @default.
• W1567873484 hasConcept C54355233 @default.
• W1567873484 hasConcept C55493867 @default.
• W1567873484 hasConcept C64943373 @default.
• W1567873484 hasConcept C71924100 @default.
• W1567873484 hasConcept C81089528 @default.
• W1567873484 hasConcept C81885089 @default.
• W1567873484 hasConcept C86803240 @default.
• W1567873484 hasConcept C95444343 @default.
• W1567873484 hasConcept C97029542 @default.
• W1567873484 hasConceptScore W1567873484C104317684 @default.
• W1567873484 hasConceptScore W1567873484C115302286 @default.
• W1567873484 hasConceptScore W1567873484C148738053 @default.
• W1567873484 hasConceptScore W1567873484C153911025 @default.
• W1567873484 hasConceptScore W1567873484C159110408 @default.
• W1567873484 hasConceptScore W1567873484C184235292 @default.
• W1567873484 hasConceptScore W1567873484C195794163 @default.
• W1567873484 hasConceptScore W1567873484C2775944032 @default.
• W1567873484 hasConceptScore W1567873484C2779963572 @default.
• W1567873484 hasConceptScore W1567873484C2781121885 @default.
• W1567873484 hasConceptScore W1567873484C49453240 @default.
• W1567873484 hasConceptScore W1567873484C54355233 @default.
• W1567873484 hasConceptScore W1567873484C55493867 @default.
• W1567873484 hasConceptScore W1567873484C64943373 @default.
• W1567873484 hasConceptScore W1567873484C71924100 @default.
• W1567873484 hasConceptScore W1567873484C81089528 @default.

• next