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- W1567892315 abstract "Microtubules at steady state show dynamic instability. This implies that two populations of microtubules exist: a high concentration of slow growers and a small concentration of fast shrinkers. Transitions between the two are possible. The rate constants of these transitions have been derived from the observations of the switching frequency of single microtubules in the microscope.It would be interesting to derive the switching kinetics from a whole population of microtubules. An attempt has been made by Caplow et al. (1988) J. B. C. 263, 10344–10352, however, using a slow temperature perturbation. We have repeated these experiments using a very fast (0.2 millisec) pressure perturbation.Equations have been derived for the decription of the relaxation process of steady state microtubules, taking into account the existence of a growth to shrinkage interconversion. These relations predict a rather small amplitude for the switching relaxation if the T.GTP or T.GDP.Pi cap is small.Experimental results show tha..." @default.
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- W1567892315 date "1991-01-01" @default.
- W1567892315 modified "2023-09-27" @default.
- W1567892315 title "A pressure jump relaxation study of microtubules showing dynamic instability at steady state" @default.
- W1567892315 doi "https://doi.org/10.1063/1.40583" @default.
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