FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1567947533> ?p ?o ?g. }

• W1567947533 endingPage "2943" @default.
• W1567947533 startingPage "2938" @default.
• W1567947533 abstract "We have studied the extent of genetic and phenotypic diversification of human immunodeficiency virus type 1 (HIV-1) upon 15 serial passages of clonal viral populations in MT-4 cell cultures. Several genetic and phenotypic modifications previously noted during evolution of HIV-1 in infected humans were also observed upon passages of the virus in cell culture. Notably, the transition from non-syncytium-inducing to syncytium-inducing phenotype (previously observed during disease progression) and fixation of amino acid substitutions at the main antigenic loop V3 of gp120 were observed in the course of replication of the virus in MT-4 cell cultures in the absence of immune selection. Interestingly, most genetic and phenotypic alterations occurred upon passage of the virus at a low multiplicity of infection (0.001 infectious particles per cell) rather than at a higher multiplicity of infection (0.1 infectious particles per cell). The degree of genetic diversification attained by HIV-1, estimated by the RNase A mismatch cleavage method and by nucleotide sequencing, is of about 0.03% of genomic sites mutated after 15 serial passages. This value is not significantly different from previous estimates for foot-and-mouth disease virus when subjected to a similar process and analysis. We conclude that several genetic and phenotypic modifications of HIV-1 previously observed in vivo occur also in the constant environment provided by a cell culture system. Dilute passage promotes in a highly significant way the expression of deviant HIV-1 genomes." @default.
• W1567947533 created "2016-06-24" @default.
• W1567947533 creator A5010609839 @default.
• W1567947533 creator A5037756418 @default.
• W1567947533 creator A5038890142 @default.
• W1567947533 creator A5039630473 @default.
• W1567947533 creator A5052489260 @default.
• W1567947533 creator A5069056857 @default.
• W1567947533 creator A5070798766 @default.
• W1567947533 date "1993-05-01" @default.
• W1567947533 modified "2023-10-18" @default.
• W1567947533 title "Dilute passage promotes expression of genetic and phenotypic variants of human immunodeficiency virus type 1 in cell culture" @default.
• W1567947533 cites W136988059 @default.
• W1567947533 cites W148067672 @default.
• W1567947533 cites W1489800389 @default.
• W1567947533 cites W1527352542 @default.
• W1567947533 cites W1553547362 @default.
• W1567947533 cites W1601537350 @default.
• W1567947533 cites W164172871 @default.
• W1567947533 cites W1685583992 @default.
• W1567947533 cites W1739595091 @default.
• W1567947533 cites W1973013569 @default.
• W1567947533 cites W1978146026 @default.
• W1567947533 cites W1983134928 @default.
• W1567947533 cites W1991945482 @default.
• W1567947533 cites W1992647629 @default.
• W1567947533 cites W2001076298 @default.
• W1567947533 cites W2001505139 @default.
• W1567947533 cites W2014317403 @default.
• W1567947533 cites W2014461116 @default.
• W1567947533 cites W2015326137 @default.
• W1567947533 cites W2019209606 @default.
• W1567947533 cites W2022291864 @default.
• W1567947533 cites W2030017358 @default.
• W1567947533 cites W2032790020 @default.
• W1567947533 cites W2033611972 @default.
• W1567947533 cites W2048574795 @default.
• W1567947533 cites W2055633360 @default.
• W1567947533 cites W2059599036 @default.
• W1567947533 cites W2069761065 @default.
• W1567947533 cites W2071135385 @default.
• W1567947533 cites W2078865263 @default.
• W1567947533 cites W2079341358 @default.
• W1567947533 cites W2084197987 @default.
• W1567947533 cites W2084341498 @default.
• W1567947533 cites W2088140730 @default.
• W1567947533 cites W2088403913 @default.
• W1567947533 cites W2097238654 @default.
• W1567947533 cites W2099286210 @default.
• W1567947533 cites W2102981176 @default.
• W1567947533 cites W2104259684 @default.
• W1567947533 cites W2114279162 @default.
• W1567947533 cites W2150956237 @default.
• W1567947533 cites W32966210 @default.
• W1567947533 cites W94921626 @default.
• W1567947533 doi "https://doi.org/10.1128/jvi.67.5.2938-2943.1993" @default.
• W1567947533 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/237623" @default.
• W1567947533 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8474182" @default.
• W1567947533 hasPublicationYear "1993" @default.
• W1567947533 type Work @default.
• W1567947533 sameAs 1567947533 @default.
• W1567947533 citedByCount "46" @default.
• W1567947533 countsByYear W15679475332012 @default.
• W1567947533 countsByYear W15679475332013 @default.
• W1567947533 countsByYear W15679475332014 @default.
• W1567947533 countsByYear W15679475332015 @default.
• W1567947533 countsByYear W15679475332016 @default.
• W1567947533 countsByYear W15679475332023 @default.
• W1567947533 crossrefType "journal-article" @default.
• W1567947533 hasAuthorship W1567947533A5010609839 @default.
• W1567947533 hasAuthorship W1567947533A5037756418 @default.
• W1567947533 hasAuthorship W1567947533A5038890142 @default.
• W1567947533 hasAuthorship W1567947533A5039630473 @default.
• W1567947533 hasAuthorship W1567947533A5052489260 @default.
• W1567947533 hasAuthorship W1567947533A5069056857 @default.
• W1567947533 hasAuthorship W1567947533A5070798766 @default.
• W1567947533 hasBestOaLocation W15679475331 @default.
• W1567947533 hasConcept C104317684 @default.
• W1567947533 hasConcept C127716648 @default.
• W1567947533 hasConcept C132351222 @default.
• W1567947533 hasConcept C140704245 @default.
• W1567947533 hasConcept C14500911 @default.
• W1567947533 hasConcept C159047783 @default.
• W1567947533 hasConcept C2522874641 @default.
• W1567947533 hasConcept C2776408679 @default.
• W1567947533 hasConcept C40479148 @default.
• W1567947533 hasConcept C54355233 @default.
• W1567947533 hasConcept C86803240 @default.
• W1567947533 hasConceptScore W1567947533C104317684 @default.
• W1567947533 hasConceptScore W1567947533C127716648 @default.
• W1567947533 hasConceptScore W1567947533C132351222 @default.
• W1567947533 hasConceptScore W1567947533C140704245 @default.
• W1567947533 hasConceptScore W1567947533C14500911 @default.
• W1567947533 hasConceptScore W1567947533C159047783 @default.
• W1567947533 hasConceptScore W1567947533C2522874641 @default.
• W1567947533 hasConceptScore W1567947533C2776408679 @default.
• W1567947533 hasConceptScore W1567947533C40479148 @default.
• W1567947533 hasConceptScore W1567947533C54355233 @default.

• next