FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1568218075> ?p ?o ?g. }

• W1568218075 endingPage "262" @default.
• W1568218075 startingPage "251" @default.
• W1568218075 abstract "Monoclonal antibody (mAb) TxE2, reactive with Toxoplasma gondii excretory products, detects an acidic 29 kDa protein (p29) which, in 2D gel electrophoresis, exhibits a migration pattern distinct from those of the toxoplasmic excretory proteins described so far. The sequence of seven peptides from tryptic digestion of isolated p29 allowed the design of primers to obtain the coding DNA sequence. The full-length gene was amplified from genomic DNA of T. gondii strain BK and the sequence was identical with that of the corresponding cDNA, providing evidence for an intron-free gene structure. A single mRNA transcript of 1.3 kb was detected by Northern blot analysis. The deduced 236 amino acid protein contains a putative N-terminal signal peptide, one site of potential N-linked glycosylation, and, close to the C-terminus, a further hydrophobic, putative transmembrane domain. With synthetic peptides spanning the sequence of p29, the epitope for mAb TxE2 was mapped adjacent to the putative signal sequence. The antigen, which represents almost 0.5% of T. gondii protein, is expressed in strains of all three intraspecies subgroups, and is associated with the parasite dense granules as demonstrated by immunoelectron microscopy. In tachyzoite-infected cells, p29 accumulates within the parasitophorous vacuole and co-localizes with its delimiting membrane. In bradyzoite-infected cells, p29 is present within the host cell cytoplasm as detected by immunofluorescence staining, and, furthermore, in the supernatant of cyst-bearing cell culture lacking extracellular parasites as shown by enzyme-linked immunosorbent assay (ELISA). Thus, p29 which is named dense granule protein (GRA)7 may indicate the presence of intracellular toxoplasma." @default.
• W1568218075 created "2016-06-24" @default.
• W1568218075 creator A5000336797 @default.
• W1568218075 creator A5031609869 @default.
• W1568218075 creator A5031993638 @default.
• W1568218075 creator A5034926293 @default.
• W1568218075 creator A5076135264 @default.
• W1568218075 date "1998-03-01" @default.
• W1568218075 modified "2023-09-25" @default.
• W1568218075 title "GRA7, an excretory 29 kDa Toxoplasma gondii dense granule antigen released by infected host cells" @default.
• W1568218075 cites W1830061030 @default.
• W1568218075 cites W1892700069 @default.
• W1568218075 cites W1911235289 @default.
• W1568218075 cites W1915965294 @default.
• W1568218075 cites W1978499269 @default.
• W1568218075 cites W1980467313 @default.
• W1568218075 cites W1986091067 @default.
• W1568218075 cites W1988412052 @default.
• W1568218075 cites W1989211609 @default.
• W1568218075 cites W2002844370 @default.
• W1568218075 cites W2006363933 @default.
• W1568218075 cites W2021767514 @default.
• W1568218075 cites W2025713936 @default.
• W1568218075 cites W2037042354 @default.
• W1568218075 cites W2037836672 @default.
• W1568218075 cites W2058050697 @default.
• W1568218075 cites W2060333964 @default.
• W1568218075 cites W2063169921 @default.
• W1568218075 cites W2063628234 @default.
• W1568218075 cites W2063844573 @default.
• W1568218075 cites W2070257791 @default.
• W1568218075 cites W2079364774 @default.
• W1568218075 cites W2082068034 @default.
• W1568218075 cites W2108342610 @default.
• W1568218075 cites W2113480696 @default.
• W1568218075 cites W2130217467 @default.
• W1568218075 cites W2137903322 @default.
• W1568218075 cites W2153249510 @default.
• W1568218075 cites W2171091522 @default.
• W1568218075 cites W2327207784 @default.
• W1568218075 cites W2411587275 @default.
• W1568218075 doi "https://doi.org/10.1016/s0166-6851(97)00227-2" @default.
• W1568218075 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9566518" @default.
• W1568218075 hasPublicationYear "1998" @default.
• W1568218075 type Work @default.
• W1568218075 sameAs 1568218075 @default.
• W1568218075 citedByCount "105" @default.
• W1568218075 countsByYear W15682180752012 @default.
• W1568218075 countsByYear W15682180752013 @default.
• W1568218075 countsByYear W15682180752014 @default.
• W1568218075 countsByYear W15682180752015 @default.
• W1568218075 countsByYear W15682180752017 @default.
• W1568218075 countsByYear W15682180752019 @default.
• W1568218075 countsByYear W15682180752020 @default.
• W1568218075 countsByYear W15682180752022 @default.
• W1568218075 crossrefType "journal-article" @default.
• W1568218075 hasAuthorship W1568218075A5000336797 @default.
• W1568218075 hasAuthorship W1568218075A5031609869 @default.
• W1568218075 hasAuthorship W1568218075A5031993638 @default.
• W1568218075 hasAuthorship W1568218075A5034926293 @default.
• W1568218075 hasAuthorship W1568218075A5076135264 @default.
• W1568218075 hasBestOaLocation W15682180751 @default.
• W1568218075 hasConcept C104317684 @default.
• W1568218075 hasConcept C10858879 @default.
• W1568218075 hasConcept C147483822 @default.
• W1568218075 hasConcept C153911025 @default.
• W1568218075 hasConcept C159654299 @default.
• W1568218075 hasConcept C167625842 @default.
• W1568218075 hasConcept C187882448 @default.
• W1568218075 hasConcept C195616568 @default.
• W1568218075 hasConcept C2778128430 @default.
• W1568218075 hasConcept C2993140218 @default.
• W1568218075 hasConcept C54355233 @default.
• W1568218075 hasConcept C55493867 @default.
• W1568218075 hasConcept C86803240 @default.
• W1568218075 hasConceptScore W1568218075C104317684 @default.
• W1568218075 hasConceptScore W1568218075C10858879 @default.
• W1568218075 hasConceptScore W1568218075C147483822 @default.
• W1568218075 hasConceptScore W1568218075C153911025 @default.
• W1568218075 hasConceptScore W1568218075C159654299 @default.
• W1568218075 hasConceptScore W1568218075C167625842 @default.
• W1568218075 hasConceptScore W1568218075C187882448 @default.
• W1568218075 hasConceptScore W1568218075C195616568 @default.
• W1568218075 hasConceptScore W1568218075C2778128430 @default.
• W1568218075 hasConceptScore W1568218075C2993140218 @default.
• W1568218075 hasConceptScore W1568218075C54355233 @default.
• W1568218075 hasConceptScore W1568218075C55493867 @default.
• W1568218075 hasConceptScore W1568218075C86803240 @default.
• W1568218075 hasIssue "2" @default.
• W1568218075 hasLocation W15682180751 @default.
• W1568218075 hasLocation W15682180752 @default.
• W1568218075 hasOpenAccess W1568218075 @default.
• W1568218075 hasPrimaryLocation W15682180751 @default.
• W1568218075 hasRelatedWork W1525670135 @default.
• W1568218075 hasRelatedWork W1786536704 @default.
• W1568218075 hasRelatedWork W1974035078 @default.
• W1568218075 hasRelatedWork W1984646339 @default.
• W1568218075 hasRelatedWork W1997568479 @default.

• next