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- W1568404022 abstract "Experiments of Lanni showed that only about 10% of the DNA of adsorbed T5 phages is rapidly transferred to the host cells; further transfer is almost halted and follows only if protein synthesis is allowed to occur. The T5-DNA molecule is known to to have several single-strand breaks at specific loci which may serve as a kind of mechanical stop in the injection process. To find out if the specific breaks on the T5-DNA have any influence on the transfer of the DNA molecule from the head through the long narrow phage tail core, we studied T5-DNA ejection triggered by T5 receptors isolated from E. coli B cells. In experiments using equilibrium density gradients we have shown that the whole T5-DNA molecule is expelled once the tail core has been opened. The specific single breaks of the T5-DNA molecule have no influence apparently on the transfer through the tail core, since no time delay in kinetic experiments was observed. More than 70% of the DNA content of receptor-bound phages was ejected in 10 min, whereas Lanni found that only 10% of the phage DNA was transferred into bacteria in the same period when 5 × 109 cells/ml were used. Zone sedimentation of a phage receptor complex through sucrose gradients showed that the whole expelled DNA is concentrated in one peak of the same sedimentation constant as the intact T5-DNA. The present results show that the first-step-transfer DNA (FST-DNA) does not exist as a separate piece in the phage particle. They suggest that Lanni's observation results from an unusual uptake mechanism by the infected cell. All these findings fit the physical model of phage DNA ejection which predicts that the whole phage DNA should be expelled once the tail core has been opened (Zarybnicky, 1969a)." @default.
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- W1568404022 date "1973-08-01" @default.
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- W1568404022 title "Infection process of T5 phages" @default.
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- W1568404022 doi "https://doi.org/10.1016/0042-6822(73)90146-3" @default.
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