FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1568574872> ?p ?o ?g. }

• W1568574872 endingPage "4426" @default.
• W1568574872 startingPage "4414" @default.
• W1568574872 abstract "Protocatechuate 2,3-dioxygenase (2,3-PCD) from Bacillus macerans JJ1b has been purified to homogeneity for the first time. The enzyme catalyzes proximal extradiol ring cleavage of protocatechuate (PCA) with the attendant incorporation of both atoms of oxygen from O2. The holoenzyme has a mass of 143 +/- 7 kDa as determined by ultracentrifugation and other techniques. It is composed of four apparently identical subunits with M(r)s of 35,500, each containing one iron atom. Mössbauer spectroscopy of 57Fe-enriched enzyme showed that the irons are indistinguishable and are high spin (S = 2) Fe2+ in both the uncomplexed and substrate-bound enzyme. However, the quadrupole splitting, delta EQ, and isomer shift, delta, of the Mössbauer spectrum changed from delta EQ = 2.57 mm/s and delta = 1.29 mm/s to delta EQ = 2.73 mm/s and delta = 1.19 mm/s upon PCA binding to the enzyme, showing that the iron environment is altered when substrate is present. The enzyme was also found to bind variable and substoichiometric amounts of Mn2+, but this metal could be removed without loss of activity or stability. The inherently electron paramagnetic resonance (EPR)-silent Fe2+ of the enzyme reversibly bound nitric oxide to produce an EPR-active species (g = 4.11, 3.95; S = 3/2). The specific activity of the enzyme was found to be correlated with the amount of the S = 3/2 species formed, showing that activity is dependent on Fe2+. Anaerobic addition of substrates to the enzyme-nitric oxide complex significantly altered the EPR spectrum, suggesting that substrates bind to or near the iron. The enzyme was inactivated by reagents that oxidize the Fe2+, such as H2O2 and K3FE(CN)6; full activity was restored after reduction of the iron by ascorbate. Steady-state kinetic data were found to be consistent with an ordered bi-uni mechanism in which the organic substrate must add to 2,3-PCD before O2. The enzyme has the broadest substrate range of any of the well-studied catecholic dioxygenases. All substrates have vicinal hydroxyl groups on the aromatic ring except 4-NH2-3-hydroxybenzoate. This is the first substrate lacking vicinal hydroxyl groups reported for catecholic extradiol dioxygenases. 2,3-PCD is the final member of the PCA dioxygenase family to be purified. It is compared with other members of this family as well as other catecholic dioxygenases." @default.
• W1568574872 created "2016-06-24" @default.
• W1568574872 creator A5019917073 @default.
• W1568574872 creator A5026384821 @default.
• W1568574872 creator A5033277278 @default.
• W1568574872 creator A5035076779 @default.
• W1568574872 creator A5036104541 @default.
• W1568574872 creator A5048542294 @default.
• W1568574872 creator A5058130714 @default.
• W1568574872 date "1993-07-01" @default.
• W1568574872 modified "2023-10-03" @default.
• W1568574872 title "Purification and characterization of protocatechuate 2,3-dioxygenase from Bacillus macerans: a new extradiol catecholic dioxygenase" @default.
• W1568574872 cites W1017515459 @default.
• W1568574872 cites W143448611 @default.
• W1568574872 cites W1482636423 @default.
• W1568574872 cites W1487351468 @default.
• W1568574872 cites W1492352549 @default.
• W1568574872 cites W1492607190 @default.
• W1568574872 cites W1495964364 @default.
• W1568574872 cites W1509366823 @default.
• W1568574872 cites W1517580958 @default.
• W1568574872 cites W1525234184 @default.
• W1568574872 cites W1525814142 @default.
• W1568574872 cites W1538749624 @default.
• W1568574872 cites W1539770653 @default.
• W1568574872 cites W1551004756 @default.
• W1568574872 cites W1553529773 @default.
• W1568574872 cites W1559495651 @default.
• W1568574872 cites W1561118044 @default.
• W1568574872 cites W1566803604 @default.
• W1568574872 cites W1567116460 @default.
• W1568574872 cites W1570510207 @default.
• W1568574872 cites W1574021748 @default.
• W1568574872 cites W1580575515 @default.
• W1568574872 cites W1588380766 @default.
• W1568574872 cites W1592830109 @default.
• W1568574872 cites W1595673006 @default.
• W1568574872 cites W1604005311 @default.
• W1568574872 cites W1628593931 @default.
• W1568574872 cites W1661458410 @default.
• W1568574872 cites W1760212501 @default.
• W1568574872 cites W1855094760 @default.
• W1568574872 cites W1903671609 @default.
• W1568574872 cites W1962707467 @default.
• W1568574872 cites W1971733876 @default.
• W1568574872 cites W1972434921 @default.
• W1568574872 cites W1984195074 @default.
• W1568574872 cites W1995559679 @default.
• W1568574872 cites W2005531646 @default.
• W1568574872 cites W2009202204 @default.
• W1568574872 cites W2010516490 @default.
• W1568574872 cites W2017103595 @default.
• W1568574872 cites W2018289835 @default.
• W1568574872 cites W2021532248 @default.
• W1568574872 cites W2025381595 @default.
• W1568574872 cites W2052131319 @default.
• W1568574872 cites W2052895859 @default.
• W1568574872 cites W2061202321 @default.
• W1568574872 cites W2075462108 @default.
• W1568574872 cites W2089242115 @default.
• W1568574872 cites W2091257376 @default.
• W1568574872 cites W2094228503 @default.
• W1568574872 cites W2100837269 @default.
• W1568574872 cites W2103235992 @default.
• W1568574872 cites W2119586900 @default.
• W1568574872 cites W2128635872 @default.
• W1568574872 cites W2133216239 @default.
• W1568574872 cites W2197482522 @default.
• W1568574872 cites W2200759734 @default.
• W1568574872 cites W2397765685 @default.
• W1568574872 cites W2414868583 @default.
• W1568574872 cites W2483646798 @default.
• W1568574872 cites W2893528626 @default.
• W1568574872 cites W4293247451 @default.
• W1568574872 doi "https://doi.org/10.1128/jb.175.14.4414-4426.1993" @default.
• W1568574872 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/204882" @default.
• W1568574872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8392511" @default.
• W1568574872 hasPublicationYear "1993" @default.
• W1568574872 type Work @default.
• W1568574872 sameAs 1568574872 @default.
• W1568574872 citedByCount "63" @default.
• W1568574872 countsByYear W15685748722012 @default.
• W1568574872 countsByYear W15685748722013 @default.
• W1568574872 countsByYear W15685748722014 @default.
• W1568574872 countsByYear W15685748722015 @default.
• W1568574872 countsByYear W15685748722016 @default.
• W1568574872 countsByYear W15685748722017 @default.
• W1568574872 countsByYear W15685748722018 @default.
• W1568574872 countsByYear W15685748722019 @default.
• W1568574872 countsByYear W15685748722020 @default.
• W1568574872 countsByYear W15685748722021 @default.
• W1568574872 crossrefType "journal-article" @default.
• W1568574872 hasAuthorship W1568574872A5019917073 @default.
• W1568574872 hasAuthorship W1568574872A5026384821 @default.
• W1568574872 hasAuthorship W1568574872A5033277278 @default.
• W1568574872 hasAuthorship W1568574872A5035076779 @default.
• W1568574872 hasAuthorship W1568574872A5036104541 @default.
• W1568574872 hasAuthorship W1568574872A5048542294 @default.

• next