FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569009165> ?p ?o ?g. }

• W1569009165 endingPage "384" @default.
• W1569009165 startingPage "380" @default.
• W1569009165 abstract "Abstract Aim To produce the H1N1 neuraminidase (NA) with catalytic activity by Pichia pastoris and screen novel inhibitors of NA from the extracts of microbial fermentation broths. Methods The truncated H1N1 NA coding gene was cloned into plasmid pPICZαA to construct the recombinant plasmid pPICZαA-NA, which was linearized and then electroporated into P. pastoris SMD1168. The correct transformants were induced by methanol for the expression of recombinant NA, which was then used to construct a high-throughput screening model for the screening of novel NA inhibitors from the extracts of fermentation broths. Results The recombinant P. pastoris could secrete the truncated NA of H1N1 with catalytic activity. Moreover, its activity was significantly inhibited by oseltamivir and zanamivir, two licensed inhibitors of H1N1 NA. Based on these, more than 20 000 microibal fermentation extracts were screened, and 6 of them showed Conclusion The truncated NA with catalytic activity could be used to construct the high-throughput model for screening new inhibitors of NA. Compared with purification from virion, the preparation of NA is more convenient and safer from P. pastoris , which would facilitate the development of high-throughput assay for screening novel NA inhibitors." @default.
• W1569009165 created "2016-06-24" @default.
• W1569009165 creator A5010422757 @default.
• W1569009165 creator A5022698041 @default.
• W1569009165 creator A5056953478 @default.
• W1569009165 creator A5082435542 @default.
• W1569009165 date "2011-09-01" @default.
• W1569009165 modified "2023-09-24" @default.
• W1569009165 title "Expression of a Truncated H1N1 Neuraminidase and High-throughput Screening of its Inhibitor" @default.
• W1569009165 cites W1757353225 @default.
• W1569009165 cites W1986051946 @default.
• W1569009165 cites W1988044361 @default.
• W1569009165 cites W1991187041 @default.
• W1569009165 cites W1994644330 @default.
• W1569009165 cites W2009428685 @default.
• W1569009165 cites W2011202054 @default.
• W1569009165 cites W2018285221 @default.
• W1569009165 cites W2042611040 @default.
• W1569009165 cites W2082581989 @default.
• W1569009165 cites W2086083638 @default.
• W1569009165 cites W2100837269 @default.
• W1569009165 cites W2138602400 @default.
• W1569009165 cites W2140566427 @default.
• W1569009165 cites W2312463092 @default.
• W1569009165 doi "https://doi.org/10.3724/sp.j.1009.2011.00380" @default.
• W1569009165 hasPublicationYear "2011" @default.
• W1569009165 type Work @default.
• W1569009165 sameAs 1569009165 @default.
• W1569009165 citedByCount "0" @default.
• W1569009165 crossrefType "journal-article" @default.
• W1569009165 hasAuthorship W1569009165A5010422757 @default.
• W1569009165 hasAuthorship W1569009165A5022698041 @default.
• W1569009165 hasAuthorship W1569009165A5056953478 @default.
• W1569009165 hasAuthorship W1569009165A5082435542 @default.
• W1569009165 hasConcept C100544194 @default.
• W1569009165 hasConcept C104317684 @default.
• W1569009165 hasConcept C134164806 @default.
• W1569009165 hasConcept C142724271 @default.
• W1569009165 hasConcept C153911025 @default.
• W1569009165 hasConcept C181199279 @default.
• W1569009165 hasConcept C185592680 @default.
• W1569009165 hasConcept C22744801 @default.
• W1569009165 hasConcept C2779134260 @default.
• W1569009165 hasConcept C2781372317 @default.
• W1569009165 hasConcept C2781442963 @default.
• W1569009165 hasConcept C3008058167 @default.
• W1569009165 hasConcept C40767141 @default.
• W1569009165 hasConcept C51323132 @default.
• W1569009165 hasConcept C524204448 @default.
• W1569009165 hasConcept C55493867 @default.
• W1569009165 hasConcept C71924100 @default.
• W1569009165 hasConcept C86803240 @default.
• W1569009165 hasConceptScore W1569009165C100544194 @default.
• W1569009165 hasConceptScore W1569009165C104317684 @default.
• W1569009165 hasConceptScore W1569009165C134164806 @default.
• W1569009165 hasConceptScore W1569009165C142724271 @default.
• W1569009165 hasConceptScore W1569009165C153911025 @default.
• W1569009165 hasConceptScore W1569009165C181199279 @default.
• W1569009165 hasConceptScore W1569009165C185592680 @default.
• W1569009165 hasConceptScore W1569009165C22744801 @default.
• W1569009165 hasConceptScore W1569009165C2779134260 @default.
• W1569009165 hasConceptScore W1569009165C2781372317 @default.
• W1569009165 hasConceptScore W1569009165C2781442963 @default.
• W1569009165 hasConceptScore W1569009165C3008058167 @default.
• W1569009165 hasConceptScore W1569009165C40767141 @default.
• W1569009165 hasConceptScore W1569009165C51323132 @default.
• W1569009165 hasConceptScore W1569009165C524204448 @default.
• W1569009165 hasConceptScore W1569009165C55493867 @default.
• W1569009165 hasConceptScore W1569009165C71924100 @default.
• W1569009165 hasConceptScore W1569009165C86803240 @default.
• W1569009165 hasIssue "5" @default.
• W1569009165 hasLocation W15690091651 @default.
• W1569009165 hasOpenAccess W1569009165 @default.
• W1569009165 hasPrimaryLocation W15690091651 @default.
• W1569009165 hasRelatedWork W2032050280 @default.
• W1569009165 hasRelatedWork W2152368084 @default.
• W1569009165 hasRelatedWork W2347300594 @default.
• W1569009165 hasRelatedWork W2350998444 @default.
• W1569009165 hasRelatedWork W2353499976 @default.
• W1569009165 hasRelatedWork W2354680849 @default.
• W1569009165 hasRelatedWork W2362026912 @default.
• W1569009165 hasRelatedWork W2368676779 @default.
• W1569009165 hasRelatedWork W2371230381 @default.
• W1569009165 hasRelatedWork W2372623480 @default.
• W1569009165 hasRelatedWork W2382198378 @default.
• W1569009165 hasRelatedWork W2383833704 @default.
• W1569009165 hasRelatedWork W2388588301 @default.
• W1569009165 hasRelatedWork W2390750833 @default.
• W1569009165 hasRelatedWork W2410484936 @default.
• W1569009165 hasRelatedWork W2440669710 @default.
• W1569009165 hasRelatedWork W2473124202 @default.
• W1569009165 hasRelatedWork W2512794384 @default.
• W1569009165 hasRelatedWork W3145811278 @default.
• W1569009165 hasRelatedWork W3197175566 @default.
• W1569009165 hasVolume "9" @default.
• W1569009165 isParatext "false" @default.
• W1569009165 isRetracted "false" @default.
• W1569009165 magId "1569009165" @default.

• next