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- W1569014305 abstract "This study was designed to evaluate the effect of decalcifying fluid types on bone tissue architecture and its staining properties following decalcification at varying temperatures. A decalcification methodology using Golding and Stewards (GS) fluid, and Jenkings fluid (JK), and a modern household microwave oven to accelerate the decalcification rate of bone tissue samples, was adopted for microscopic investigations at room temperature, 30 0 C, 40 0 C, and 50 0 C respectively. Bone biopsy was obtained from a rabbit’s limb and fixed immediately for 24hrs in 10% formalin. The result showed that micrographs from bone tissue sections decalcified with 5% GS presented excellent histological architecture when compared with those decalcified with 5% JK at 30 0 C while 5% JK presented good histological architecture when compared with those decalcified with 5% GS at 40 0 C.Severe cytoarchitectural distortion was observed for bone tissues decalcified with 5% GS at 50 0 C, while bone tissues decalcified with 5% JK at 50 0 C, presented cyto-architectural distortions that were however, not as severe as those of 5% GS at 50 0 C. Our findings suggest therefore, that decalcification with 5% GS at 30 0 C, comparatively gives optimal histological results and preserves the tissues’ best staining properties. Key words: microwave oven, temperatures, bone decalcification, Histology," @default.
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- W1569014305 date "2012-01-01" @default.
- W1569014305 modified "2023-09-26" @default.
- W1569014305 title "Microwave oven-induced decalcification at varying temperatures: A comparative study of two decalcifying fluids" @default.
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