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• W1569062257 startingPage "2683" @default.
• W1569062257 abstract "Abstract Background 4‐α‐Glucanotransferases are useful enzymes to modify starch owing to their transglycosylation activity. In this study, codon optimizations were conducted to overproduce a thermostable 4‐α‐glucanotransferase from Thermus thermophilus ( TT α GT ) . Results Two variants, termed TT α GT‐P4CCG and TT α GT ‐mut6, were constructed, which have the optimized codon at the first rare codon and optimized codons at all six chosen rare codons at the N‐terminus of TT α GT , respectively. In the Escherichia coli system, the expression of both optimized genes was enhanced by about 100‐fold relative to that of the original gene, whereas all six mutated codons contributed to the overall enhancement of TT α GT production in Bacillus subtilis . On the basis of the α GTase activity of the crude cell extracts, relative activities of 1:2.9:5.8 were determined for TT α GT , TT α GT‐P4CCG and TT α GT ‐mut6, respectively, in B. subtilis . In addition, the activity of TT α GT ‐mut6 from B. subtilis grown without antibiotics was as much as that with the antibiotics. Finally, after heat treatment, the specific activity of TT α GT ‐mut6 from B. subtilis was 1.5‐fold greater than that from E. coli . Conclusion The codon‐optimized TT α GT that was produced in a GRAS microorganism, B. subtilis , without the selection antibiotics is potentially useful in the food industry as a food‐grade enzyme. © 2013 Society of Chemical Industry" @default.
• W1569062257 created "2016-06-24" @default.
• W1569062257 creator A5017180454 @default.
• W1569062257 creator A5042206655 @default.
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• W1569062257 date "2013-04-26" @default.
• W1569062257 modified "2023-10-18" @default.
• W1569062257 title "Overproduction of a thermostable 4-α-glucanotransferase by codon optimization at N-terminus region" @default.
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• W1569062257 doi "https://doi.org/10.1002/jsfa.6084" @default.
• W1569062257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23620355" @default.
• W1569062257 hasPublicationYear "2013" @default.
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