Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569071713> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W1569071713 endingPage "1003" @default.
- W1569071713 startingPage "991" @default.
- W1569071713 abstract "Enzymatic N,O-acyltransfer of carcinogenic N-arylhydroxamic acids such as N-droxy-2-acetylaminofluorene (N-OH-AAF) results in the production of reactive electrophiles that can bond covalently with nucleophiles and also can cause inactivation of acyltransferase activity in a mechanismbased manner. Incubation of partially purified rat hepatic N-acetyltransferases (NAT) with N-OH-AAF resulted in extensive inactivation of N-OH-AAF/4-aminoazobenzene (AAB) N, N-acetyltransferase and acetyl coenzyme A (AcCoA)/procainamide (PA) N-acetyltransferase activities, whereas AcCoA/p-aminobenzoic acid (PABA) N-acetyltransferase activity was inhibited only slightly. Affinity chromatography with Sepharose 6B 2-aminofluorene (2-AF) resulted in the separation of two NAT activities. NAT I primarily catalyzed the AcCoA-dependent acetylation of PABA; NAT II catalyzed, N, N-acetyltransfer(N-OH-AAF/AAB), AcCoA/PA N-acetyltransfer and N-OH-AAF N,O-acyltransfer (AHAT) activities. Most of the AcCoA/2-AF N-acetyltransferase activity eluted in the NAT II fraction. Results of inactivation experiments with N-OH-AAF and the NAT II fractions suggested that one NAT isozyme was responsible for catalyzing the N-OH-AAF/AAB, AcCoA/PA and N,O-acyltransfer reactions and that inactivation of NAT II correlated with the extent of covalent binding to protein. Further purification of the NAT II fractions by chromatofocusing resulted in a 1300-fold purification of the N-OH-AAF/AAB activity and the coelution of N-OH-AAF/AAB, AcCoA/PA and N, O-acyltransferase activities. These studies indicate that N,O-acyltransfer, arylhydroxamic acid-dependent N-acetylation of arylamines (N,N-acetyltransfer), and AcCoA-dependent N-acetylation of PA may be catalyzed by a common enzyme in rat liver, whereas a second enzyme is responsible for the AcCoAdependent N-acetylation of PABA." @default.
- W1569071713 created "2016-06-24" @default.
- W1569071713 creator A5040579052 @default.
- W1569071713 creator A5072723272 @default.
- W1569071713 date "1990-03-01" @default.
- W1569071713 modified "2023-10-16" @default.
- W1569071713 title "Bioactivation of N-arylhydroxamic acids by rat hepatic N-acetyltransferase" @default.
- W1569071713 cites W145821160 @default.
- W1569071713 cites W1550542709 @default.
- W1569071713 cites W1578782925 @default.
- W1569071713 cites W1775749144 @default.
- W1569071713 cites W1884410227 @default.
- W1569071713 cites W1968252448 @default.
- W1569071713 cites W1991588891 @default.
- W1569071713 cites W1991926100 @default.
- W1569071713 cites W2008232320 @default.
- W1569071713 cites W2014829116 @default.
- W1569071713 cites W2033790626 @default.
- W1569071713 cites W2041280166 @default.
- W1569071713 cites W2048800047 @default.
- W1569071713 cites W2097280465 @default.
- W1569071713 cites W2135718455 @default.
- W1569071713 cites W2283438955 @default.
- W1569071713 cites W2328357453 @default.
- W1569071713 cites W2336832114 @default.
- W1569071713 cites W986959249 @default.
- W1569071713 cites W1987186326 @default.
- W1569071713 doi "https://doi.org/10.1016/0006-2952(90)90277-r" @default.
- W1569071713 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2322300" @default.
- W1569071713 hasPublicationYear "1990" @default.
- W1569071713 type Work @default.
- W1569071713 sameAs 1569071713 @default.
- W1569071713 citedByCount "21" @default.
- W1569071713 crossrefType "journal-article" @default.
- W1569071713 hasAuthorship W1569071713A5040579052 @default.
- W1569071713 hasAuthorship W1569071713A5072723272 @default.
- W1569071713 hasConcept C104317684 @default.
- W1569071713 hasConcept C119157956 @default.
- W1569071713 hasConcept C178790620 @default.
- W1569071713 hasConcept C180577832 @default.
- W1569071713 hasConcept C181199279 @default.
- W1569071713 hasConcept C182516595 @default.
- W1569071713 hasConcept C185592680 @default.
- W1569071713 hasConcept C2775970874 @default.
- W1569071713 hasConcept C2778328694 @default.
- W1569071713 hasConcept C2778340702 @default.
- W1569071713 hasConcept C2778470496 @default.
- W1569071713 hasConcept C2779649592 @default.
- W1569071713 hasConcept C2910274206 @default.
- W1569071713 hasConcept C31258907 @default.
- W1569071713 hasConcept C41008148 @default.
- W1569071713 hasConcept C55493867 @default.
- W1569071713 hasConcept C71240020 @default.
- W1569071713 hasConceptScore W1569071713C104317684 @default.
- W1569071713 hasConceptScore W1569071713C119157956 @default.
- W1569071713 hasConceptScore W1569071713C178790620 @default.
- W1569071713 hasConceptScore W1569071713C180577832 @default.
- W1569071713 hasConceptScore W1569071713C181199279 @default.
- W1569071713 hasConceptScore W1569071713C182516595 @default.
- W1569071713 hasConceptScore W1569071713C185592680 @default.
- W1569071713 hasConceptScore W1569071713C2775970874 @default.
- W1569071713 hasConceptScore W1569071713C2778328694 @default.
- W1569071713 hasConceptScore W1569071713C2778340702 @default.
- W1569071713 hasConceptScore W1569071713C2778470496 @default.
- W1569071713 hasConceptScore W1569071713C2779649592 @default.
- W1569071713 hasConceptScore W1569071713C2910274206 @default.
- W1569071713 hasConceptScore W1569071713C31258907 @default.
- W1569071713 hasConceptScore W1569071713C41008148 @default.
- W1569071713 hasConceptScore W1569071713C55493867 @default.
- W1569071713 hasConceptScore W1569071713C71240020 @default.
- W1569071713 hasIssue "6" @default.
- W1569071713 hasLocation W15690717131 @default.
- W1569071713 hasLocation W15690717132 @default.
- W1569071713 hasOpenAccess W1569071713 @default.
- W1569071713 hasPrimaryLocation W15690717131 @default.
- W1569071713 hasRelatedWork W1569071713 @default.
- W1569071713 hasRelatedWork W1945069805 @default.
- W1569071713 hasRelatedWork W2061203773 @default.
- W1569071713 hasRelatedWork W2074330397 @default.
- W1569071713 hasRelatedWork W2118375848 @default.
- W1569071713 hasRelatedWork W2134026908 @default.
- W1569071713 hasRelatedWork W2151443151 @default.
- W1569071713 hasRelatedWork W2393649261 @default.
- W1569071713 hasRelatedWork W2618996160 @default.
- W1569071713 hasRelatedWork W4360918702 @default.
- W1569071713 hasVolume "39" @default.
- W1569071713 isParatext "false" @default.
- W1569071713 isRetracted "false" @default.
- W1569071713 magId "1569071713" @default.
- W1569071713 workType "article" @default.