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- W1569073051 abstract "Publisher Summary This chapter focuses on the interaction of glycosphingolipids with signal transducers and membrane proteins in glycosphingolipid-enriched microdomains (GEMs), and describes the isolation, chemical composition, and functional characterization of GEMs or glycosynapses from a few types of cells. The general procedure for preparation of GEMs is discussed. Two conditions are used for the lysis and homogenization of cells: (i) in a medium containing 1% Triton X-100 and (ii) in hypertonic sodium carbonate medium followed by sucrose density gradient ultracentrifugation to separate low-density buoyant fraction from high-density soluble fraction. The chapter also discusses the interaction of GalNAcDSLc 4 ganglioside with cSrc and RhoA in GEM from the renal cell carcinoma cell line TOS1; the interaction of GalNAcDSLc 4 and signal transducer molecules from the GEM Fraction of TOS1 cells; the coordinated interaction of GM3 with cSrc and Csk the physiological inhibitor of cSrc,in Neuro2a cells; and the interaction of GM3 ganglioside with tetraspanin CD9/Integrin α3 complex in ldlD/CD9 cells." @default.
- W1569073051 created "2016-06-24" @default.
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- W1569073051 date "2003-01-01" @default.
- W1569073051 modified "2023-09-29" @default.
- W1569073051 title "Interaction of Glycosphingolipids with Signal Transducers and Membrane Proteins in Glycosphingolipid-Enriched Microdomains" @default.
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- W1569073051 doi "https://doi.org/10.1016/s0076-6879(03)01052-8" @default.
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