Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569078601> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1569078601 endingPage "259" @default.
- W1569078601 startingPage "252" @default.
- W1569078601 abstract "Abstract Increasing demand and overfishing of high‐value species has promoted interest in both conservation and aquaculture initiatives supporting stock restoration programs for tropical sea cucumbers. Accordingly, there is a need for baseline information on the genetic structures and relatedness of sea cucumber populations to support sustainable implementation of mariculture and conservation programs, which often involve coastal communities in developing countries. Identification of a non‐destructive tissue sampling technique for sea cucumbers that allows extraction of high‐quality genomic DNA in a sustainable and culturally appropriate way is thus required. Six sampling techniques were assessed for their suitability to collect tissue for DNA extraction from sandfish ( Holothuria scabra ): core needle biopsy, punch biopsy, shave biopsy, buccal swab, anal swab, and evisceration. The quantity, quality, and purity of extracted DNA were compared to assess the relative merit of each sampling method. The swab biopsy method produced the best quality DNA agarose band image, and evisceration resulted in the highest yields of DNA , at an average of 525.9 (±98.0) μg g −1 . However, when considering all criteria assessed, the swab biopsy methods (both buccal and anal) proved superior. Swabbing not only produced the best quality DNA agarose band image, it was also the only technique that produced DNA that amplified 100% of the time across both extraction protocols. We hope that the non‐destructive sampling techniques evaluated in this study provide a foundation for the genetic analysis of sea cucumber stocks to support their conservation and management." @default.
- W1569078601 created "2016-06-24" @default.
- W1569078601 creator A5011305538 @default.
- W1569078601 creator A5018141930 @default.
- W1569078601 creator A5025849811 @default.
- W1569078601 date "2015-08-06" @default.
- W1569078601 modified "2023-10-03" @default.
- W1569078601 title "A non-destructive tissue sampling technique for holothurians to facilitate extraction of DNA for genetic analysis" @default.
- W1569078601 cites W1506650257 @default.
- W1569078601 cites W1514825644 @default.
- W1569078601 cites W1584313779 @default.
- W1569078601 cites W1810869755 @default.
- W1569078601 cites W1924106145 @default.
- W1569078601 cites W1979468621 @default.
- W1569078601 cites W1987346078 @default.
- W1569078601 cites W2000591230 @default.
- W1569078601 cites W2002873355 @default.
- W1569078601 cites W2007032317 @default.
- W1569078601 cites W2012941279 @default.
- W1569078601 cites W2013685141 @default.
- W1569078601 cites W2020867650 @default.
- W1569078601 cites W2023858100 @default.
- W1569078601 cites W2031793996 @default.
- W1569078601 cites W2042322234 @default.
- W1569078601 cites W2049559502 @default.
- W1569078601 cites W2051890931 @default.
- W1569078601 cites W2063714552 @default.
- W1569078601 cites W2064997131 @default.
- W1569078601 cites W2074745339 @default.
- W1569078601 cites W2081858203 @default.
- W1569078601 cites W2083566486 @default.
- W1569078601 cites W2087140567 @default.
- W1569078601 cites W2093489259 @default.
- W1569078601 cites W2102133138 @default.
- W1569078601 cites W2105762979 @default.
- W1569078601 cites W2106792439 @default.
- W1569078601 cites W2110869990 @default.
- W1569078601 cites W2116505001 @default.
- W1569078601 cites W2144502483 @default.
- W1569078601 cites W2152378712 @default.
- W1569078601 cites W2164718269 @default.
- W1569078601 cites W2166258948 @default.
- W1569078601 cites W2407017980 @default.
- W1569078601 cites W4253495628 @default.
- W1569078601 doi "https://doi.org/10.1111/ivb.12091" @default.
- W1569078601 hasPublicationYear "2015" @default.
- W1569078601 type Work @default.
- W1569078601 sameAs 1569078601 @default.
- W1569078601 citedByCount "4" @default.
- W1569078601 countsByYear W15690786012017 @default.
- W1569078601 countsByYear W15690786012021 @default.
- W1569078601 countsByYear W15690786012023 @default.
- W1569078601 crossrefType "journal-article" @default.
- W1569078601 hasAuthorship W1569078601A5011305538 @default.
- W1569078601 hasAuthorship W1569078601A5018141930 @default.
- W1569078601 hasAuthorship W1569078601A5025849811 @default.
- W1569078601 hasConcept C104317684 @default.
- W1569078601 hasConcept C150903083 @default.
- W1569078601 hasConcept C18903297 @default.
- W1569078601 hasConcept C2776093933 @default.
- W1569078601 hasConcept C49105822 @default.
- W1569078601 hasConcept C505870484 @default.
- W1569078601 hasConcept C54355233 @default.
- W1569078601 hasConcept C78063203 @default.
- W1569078601 hasConcept C86803240 @default.
- W1569078601 hasConceptScore W1569078601C104317684 @default.
- W1569078601 hasConceptScore W1569078601C150903083 @default.
- W1569078601 hasConceptScore W1569078601C18903297 @default.
- W1569078601 hasConceptScore W1569078601C2776093933 @default.
- W1569078601 hasConceptScore W1569078601C49105822 @default.
- W1569078601 hasConceptScore W1569078601C505870484 @default.
- W1569078601 hasConceptScore W1569078601C54355233 @default.
- W1569078601 hasConceptScore W1569078601C78063203 @default.
- W1569078601 hasConceptScore W1569078601C86803240 @default.
- W1569078601 hasIssue "3" @default.
- W1569078601 hasLocation W15690786011 @default.
- W1569078601 hasOpenAccess W1569078601 @default.
- W1569078601 hasPrimaryLocation W15690786011 @default.
- W1569078601 hasRelatedWork W1571238902 @default.
- W1569078601 hasRelatedWork W2088073763 @default.
- W1569078601 hasRelatedWork W2351051559 @default.
- W1569078601 hasRelatedWork W2500998126 @default.
- W1569078601 hasRelatedWork W2551934689 @default.
- W1569078601 hasRelatedWork W2886978591 @default.
- W1569078601 hasRelatedWork W2991449745 @default.
- W1569078601 hasRelatedWork W3121479699 @default.
- W1569078601 hasRelatedWork W4306377508 @default.
- W1569078601 hasRelatedWork W88188735 @default.
- W1569078601 hasVolume "134" @default.
- W1569078601 isParatext "false" @default.
- W1569078601 isRetracted "false" @default.
- W1569078601 magId "1569078601" @default.
- W1569078601 workType "article" @default.