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• W1569093838 abstract "Supra-normal temperatures have been shown to induce reversible ultrastructural lesions in cultured cells. These lesions involve the nucleolus and are characterized by configurational changes in the nucleolar ribonucleoproteins. A correlative ultrastructural and biochemical study has been carried out on ascites tumor cells in an attempt to establish a relationship between the ultrastructural and functional alterations. The present report describes the ultrastructural modifications caused by a thermic shock as studied by complementary methods of enzymic extraction, high resolution radioautography and biochemistry. Nucleoli of ascites tumor cells incubated at 44.5 °C for 30 min display a disappearance of the granular form of their ribonucleoproteins associated with a retraction of intranucleolar chromatin. Only closely packed fibrillar ribonucleoproteins are left. Kinetic biochemical and ultrastructural studies at 44.5 °C shows that the retraction of intranucleolar chromatin precedes the configurational changes of the granular ribonucleoproteins and is associated with a rapid decrease of the specific activity of nucleolar RNA. When cells are incubated for 30 min at temperatures varying from 37 °C to 45 °C, the intranucleolar portion of the chromatin disappears at 43 °C and the granular ribonucleoproteins at 44.5 °C; the maximum decrease of the specific activity of nucleolar RNA is then observed around 41–43 °C. High resolution radioautography confirms the absence of incorporation of tritiated uridine in heated cells. If the incorporation precedes the thermic shock, radioactivity accumulates in the nucleolus and is not migrating into the cytoplasm; localized nucleolar labeling can be seen if the labeled uridine is added at the beginning of the shock. The results indicate that the synthesis of nucleolar RNA is heat-sensitive and related to the presence of intranucleolar chromatin. It is suggested that the granular ribonucleoproteins are transitory configurational forms, unraveling after a thermic shock. They might be associated with the transport of RNA from the nucleolus to the cytoplasm. The fibrillar ribonucleoproteins appear to be a more stable form in which the nucleolar RNA could be “pooled”." @default.
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• W1569093838 date "1969-06-01" @default.
• W1569093838 modified "2023-10-14" @default.
• W1569093838 title "Effet de la température supra-optimale sur les ribonucléoprotéines et le RNA nucléolaire" @default.
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• W1569093838 doi "https://doi.org/10.1016/0014-4827(69)90570-9" @default.
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