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- W156913516 abstract "The plantain bananas are among the most valued crop plants in thetropical world. However, the commercially attainable yields are very lowcompared to dessert bananas. Conventional breeding of this plant remains adifficult endeavor because of high sterility and polyploidy; therefore, theintegration of biotechnology into plantain improvement programmes isessential. Somatic embryogenesis has the great potential for rapid and efficientregeneration of plantlets and offers opportunities for large-scale production ofplant material. The yield and quality of somatic embryos produced in cellculture depends on the media constituents and explants type. In this study, invitro rhizomes initiated from shoot tips (8 week-old cultures) of cultivarsNangka and Tanduk and immature male flowers of cultivar Nangka were usedas explants. The results of callus initiation showed that treatments with 2,4-D,Picloram and Dicamba produced callus ranging from 0.0-82.0% for cultivar Nangka and 0.0-58.0% for cultivar Tanduk. The type of callus varieddepending on concentrations of PGRs used for the initiation. However, nocallus formation was obtained from the auxin-free medium. The study on thedetermination of callus growth curve showed that treatments with 2,4-D andPicloram for both cultivars Nangka and Tanduk produced a sigmoidal pattern,except for several concentrations which failed to show the callus growthpattern. Whereas, for the Dicamba treatments, there was no callus growth at all.The effects of two basal media supplemented with 2,4-D and Picloram atdifferent concentrations were studied. The highest callus fresh weight (0.37 g)was attained on SH medium plus 2.5 µM Picloram. The effect of 2,4-D andPicloram in combination with other auxins and cytokinins, showed that therewas no constant trend on callus growth in response to the treatments. However,the treatments using 2,4-D and Picloram in combination with other cytokininsproduced nodular and compact callus. The callus produced embryogenicstructure (13.3-66.7%) in the somatic embryogenesis medium, but no shootregeneration was achieved, instead only roots were formed. In the initiation ofcallus from immature male flowers, 49.7% of flower cluster responded to formglobular embryogenic callus and the highest response was from flower of rows12 and 13 (60%). The study on the development of transformation protocol byparticle bombardment using in vitro rhizome slices and embryogenic callus asexplants and GFP fluorescent gene as the reporter gene revealed an unstabletransient expression in the cell." @default.
- W156913516 created "2016-06-24" @default.
- W156913516 creator A5000584901 @default.
- W156913516 date "2001-12-01" @default.
- W156913516 modified "2023-09-24" @default.
- W156913516 title "Callus formation, somatic embryogenesis and development of transformation protocol by practicle bombardment in plantain banana" @default.
- W156913516 hasPublicationYear "2001" @default.
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