Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569138356> ?p ?o ?g. }
- W1569138356 endingPage "336" @default.
- W1569138356 startingPage "326" @default.
- W1569138356 abstract "A strategy to circumvent immune responses to adenovirus (Ad) resulting from natural infection or repeated vector administrations involves sequential use of vectors from different Ad serotypes. To further develop an Ad-HIV recombinant AIDS vaccine approach, a replication-defective recombinant Ad from a non-subgroup C virus was required. Using a cosmid system, we generated an Ad7ΔE1ΔE3HIVMN env/rev recombinant virus and compared expression of the inserted HIV genes with a similarly constructed replication-competent Ad7ΔE3HIVMNenv/rev recombinant. Ad7ΔE1ΔE3HIVMNenv/rev expressed both HIV env and rev gene products. The envelope protein was correctly processed and functional, mediating syncytia formation of Ad7ΔE1ΔE3HIVMN env/rev-infected cells and CD4+ T lymphocytes. Ad7ΔE1ΔE3HIVMNenv/rev could be amplified on 293-ORF6 cells, containing the E4 ORF6 gene, shown earlier to support production of an Ad7 vector lacking the E1a gene. The utility of this cell line is now extended to the production of replication-defective Ad7 recombinants lacking E1a, E1b, and protein IX genes. Sequential immunizations with Ad-HIV recombinants based in different Ad serotypes have been shown to effectively elicit both humoral and cellular HIV-specific immune responses. The recombinant Ad7ΔE1ΔE3HIVMNenv/rev will be useful in such AIDS vaccine strategies. Further, these studies have created new cosmid vectors that can be applied to generation of single- or double-deleted Ad7 recombinants with foreign genes inserted into the E1 and/or E3 regions." @default.
- W1569138356 created "2016-06-24" @default.
- W1569138356 creator A5035934164 @default.
- W1569138356 creator A5045929439 @default.
- W1569138356 creator A5067964875 @default.
- W1569138356 creator A5071194725 @default.
- W1569138356 creator A5076709492 @default.
- W1569138356 creator A5078241211 @default.
- W1569138356 creator A5086424576 @default.
- W1569138356 date "2003-02-01" @default.
- W1569138356 modified "2023-09-27" @default.
- W1569138356 title "Development of an Ad7 cosmid system and generation of an Ad7ΔE1ΔE3HIVMN env/rev recombinant virus" @default.
- W1569138356 cites W1531311762 @default.
- W1569138356 cites W1576644054 @default.
- W1569138356 cites W1584836554 @default.
- W1569138356 cites W1778865595 @default.
- W1569138356 cites W1965606294 @default.
- W1569138356 cites W1966584188 @default.
- W1569138356 cites W1967446284 @default.
- W1569138356 cites W1967937424 @default.
- W1569138356 cites W1972244141 @default.
- W1569138356 cites W1973957725 @default.
- W1569138356 cites W1984183779 @default.
- W1569138356 cites W1986693333 @default.
- W1569138356 cites W1986868355 @default.
- W1569138356 cites W1989085325 @default.
- W1569138356 cites W2004200027 @default.
- W1569138356 cites W2010023095 @default.
- W1569138356 cites W2012106170 @default.
- W1569138356 cites W2012613745 @default.
- W1569138356 cites W2017514564 @default.
- W1569138356 cites W2019725379 @default.
- W1569138356 cites W2031583162 @default.
- W1569138356 cites W2039431795 @default.
- W1569138356 cites W2043647107 @default.
- W1569138356 cites W2044296969 @default.
- W1569138356 cites W2046782578 @default.
- W1569138356 cites W2048301060 @default.
- W1569138356 cites W2051108441 @default.
- W1569138356 cites W2062144971 @default.
- W1569138356 cites W2068831259 @default.
- W1569138356 cites W2075050600 @default.
- W1569138356 cites W2077633291 @default.
- W1569138356 cites W2084437187 @default.
- W1569138356 cites W2085947327 @default.
- W1569138356 cites W2090256161 @default.
- W1569138356 cites W2093578279 @default.
- W1569138356 cites W2093697903 @default.
- W1569138356 cites W2098062170 @default.
- W1569138356 cites W2098431648 @default.
- W1569138356 cites W2108533270 @default.
- W1569138356 cites W2111759310 @default.
- W1569138356 cites W2115675746 @default.
- W1569138356 cites W2117842784 @default.
- W1569138356 cites W2122822515 @default.
- W1569138356 cites W2126626052 @default.
- W1569138356 cites W2127481689 @default.
- W1569138356 cites W2129484395 @default.
- W1569138356 cites W2131095976 @default.
- W1569138356 cites W2133352354 @default.
- W1569138356 cites W2140423552 @default.
- W1569138356 cites W2144563230 @default.
- W1569138356 cites W2156487862 @default.
- W1569138356 cites W2159448504 @default.
- W1569138356 cites W2160690575 @default.
- W1569138356 cites W2165226718 @default.
- W1569138356 cites W2217492872 @default.
- W1569138356 cites W2313269464 @default.
- W1569138356 cites W2409243771 @default.
- W1569138356 cites W4230774644 @default.
- W1569138356 doi "https://doi.org/10.1038/sj.gt.3301903" @default.
- W1569138356 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12595891" @default.
- W1569138356 hasPublicationYear "2003" @default.
- W1569138356 type Work @default.
- W1569138356 sameAs 1569138356 @default.
- W1569138356 citedByCount "20" @default.
- W1569138356 countsByYear W15691383562012 @default.
- W1569138356 countsByYear W15691383562013 @default.
- W1569138356 countsByYear W15691383562014 @default.
- W1569138356 countsByYear W15691383562016 @default.
- W1569138356 countsByYear W15691383562017 @default.
- W1569138356 crossrefType "journal-article" @default.
- W1569138356 hasAuthorship W1569138356A5035934164 @default.
- W1569138356 hasAuthorship W1569138356A5045929439 @default.
- W1569138356 hasAuthorship W1569138356A5067964875 @default.
- W1569138356 hasAuthorship W1569138356A5071194725 @default.
- W1569138356 hasAuthorship W1569138356A5076709492 @default.
- W1569138356 hasAuthorship W1569138356A5078241211 @default.
- W1569138356 hasAuthorship W1569138356A5086424576 @default.
- W1569138356 hasBestOaLocation W15691383561 @default.
- W1569138356 hasConcept C10389963 @default.
- W1569138356 hasConcept C104317684 @default.
- W1569138356 hasConcept C159047783 @default.
- W1569138356 hasConcept C2522874641 @default.
- W1569138356 hasConcept C35101798 @default.
- W1569138356 hasConcept C39060996 @default.
- W1569138356 hasConcept C40767141 @default.
- W1569138356 hasConcept C54355233 @default.