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• W1569146283 abstract "Human opioid receptors of the δ, µ and κ subtypes were successfully expressed in Escherichia coli as fusions to the C‐terminus of the periplasmic maltose‐binding protein, MBP. Expression levels of correctly folded receptor molecules were comparable for the three subtypes and reached an average of 30 receptors·cell −1 or 0.5 pmol·mg −1 membrane protein. Binding of [ 3 H]diprenorphine to intact cells or membrane preparations was saturatable, with a dissociation constant, K D , of 2.5 n m , 0.66 n m and 0.75 n m for human δ, µ and κ opioid receptors (hDOR, hMOR and hKOR, respectively). Recombinant receptors of the three subtypes retained selectivity and nanomolar affinity for their specific antagonists. Agonist affinities were decreased by one to three orders of magnitude as compared to values measured for receptors expressed in mammalian cells. The effect of sodium on agonist binding to E. coli ‐expressed receptors was investigated. Receptor high‐affinity state for agonists was reconstituted in the presence of heterotrimeric G proteins. We also report affinity values of endomorphins 1 and 2 for µ opioid receptors expressed both in E. coli and in COS cells. Our results confirm that opioid receptors can be expressed in a functional form in bacteria and point out the advantages of E. coli as an expression system for pharmacological studies." @default.
• W1569146283 created "2016-06-24" @default.
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• W1569146283 date "1999-03-01" @default.
• W1569146283 modified "2023-10-17" @default.
• W1569146283 title "Expression of δ, κ and µ human opioid receptors in<i>Escherichia coli</i>and reconstitution of the high-affinity state for agonist with heterotrimeric G proteins" @default.
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• W1569146283 doi "https://doi.org/10.1046/j.1432-1327.1999.00187.x" @default.
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