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- W1569176600 abstract "Abstract : The work described in this report is based on discussion with Dr. Linda Chrisey following the catastrophic loss of reagents and samples as a consequence of the effects of Hurricane Katrina. The work is a full departure from the previously approved and funded research, which for both practical reasons (related to loss of reagents as a result of the hurricane), and for programmatic reasons (reflecting changes in personnel and reduced interest in the previously approved research aimed at development and evaluation of a DNA vaccine for E. rhusiopathiae). The research represents the most feasible, as well as the most useful, expenditure of funds and research effort, since it capitalizes on research techniques and products developed in previous years of funding. Objective 1: Use of hybridoma tissue culture batch reactors to produce monoclonal antibodies from existing hybridomas specific for Tursiops truncatus immunoglobulin isotypes IgG (combined reactivity for IgGl and IgG2) and IgA in quantities adequate to conduct quantitative assays of serum samples of interest. Objective 2: Purification of T. truncatus IgGl and IgG2 using Protein G and thiophilic affinity columns Objective 3: Purification of T. truncatus IgM using Sephacryl S-300HR Objective 4: Production of monoclonal antibodies specific for T. truncatus gamma 1, gamma2, and mu heavy chains Objective 5: Production of polyclonal antiserum against T. truncatus IgA, IgM, and IgG (combined) Objective 6: Statistical analysis of samples assayed by this laboratory." @default.
- W1569176600 created "2016-06-24" @default.
- W1569176600 creator A5074887823 @default.
- W1569176600 date "2007-02-08" @default.
- W1569176600 modified "2023-09-24" @default.
- W1569176600 title "Production of a DNA Vaccine Specific for the 64 kDa Protective Antigen of Erysipelothrix rhusiopathiae" @default.
- W1569176600 doi "https://doi.org/10.21236/ada462832" @default.
- W1569176600 hasPublicationYear "2007" @default.
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