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• W1569182295 startingPage "1189" @default.
• W1569182295 abstract "BHK cells infected with defective-interfering passages of Sindbis virus accumulate a species of RNA (20 S ) that is about half the molecular weight of the major viral mRNA (26 S ). We have performed competitive hybridization experiments with these species of RNA and have established that 20 S RNA contains approximately 50% of the nucleotide sequences present in 26 S RNA. Our further studies, however, demonstrate that 20 S RNA is unable to carry out the messenger function of 26 S RNA. We found very little of the defective RNA associated with polysomes in vivo. In addition, it was unable to stimulate protein synthesis in vitro under conditions in which 26 S RNA was translated. We have also examined viral RNA synthesis in BHK cells infected with standard or defective-interfering passages of Sindbis virus. This comparison suggests that defective partioles do not synthesize a functional replicase." @default.
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• W1569182295 date "1974-11-01" @default.
• W1569182295 modified "2023-09-26" @default.
• W1569182295 title "Defective Interfering Passages of Sindbis Virus: Nature of the Intracellular Defective Viral RNA" @default.
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• W1569182295 doi "https://doi.org/10.1128/jvi.14.5.1189-1198.1974" @default.
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