FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569200690> ?p ?o ?g. }

• W1569200690 endingPage "6231" @default.
• W1569200690 startingPage "6223" @default.
• W1569200690 abstract "pKM101 is a naturally occurring plasmid that carries mucAB, an analog of the umuDC operon, the gene products of which are required for the SOS-dependent processing of damaged DNA necessary for most mutagenesis. Genetic studies have indicated that mucAB expression is controlled by the SOS regulatory circuit, with LexA acting as a direct repressor. pGW16 is a pKM101 derivative obtained by N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis that was originally identified on the basis of its ability to cause a modest increase in spontaneous mutation rate. In this report, we show that pGW16 differs from pKM101 in being able to enhance methyl methanesulfonate mutagenesis and to confer substantial resistance to UV killing in a lexA3 host. The mutation carried by pGW16 is dominant and was localized to a 2.4-kb region of pGW16 that includes the mucAB coding region and approximately 0.6 kb of the 5'-flanking region. We determined the sequence of a 119-bp fragment containing the region upstream of mucAB and identified a single-base-pair change in that region, a G.C-to-A.T transition that alters a sequence homologous to known LexA-binding sites. DNA gel shift experiments indicate that LexA protein binds poorly to a 125-bp fragment containing this mutation, whereas a fragment containing the wild-type sequence is efficiently bound by LexA. This mutation also alters an overlapping sequence that is homologous to the -10 region of Escherichia coli promoters, moving it closer to the consensus sequence. The observation that the synthesis of pGW16-encoded mucAB proteins in maxicells is increased relative to that of pKM101-encoded mucAB proteins even in the absence of a lexA+ plasmid suggests that this mutation also increases the activity of the mucAB promoter." @default.
• W1569200690 created "2016-06-24" @default.
• W1569200690 creator A5037692690 @default.
• W1569200690 creator A5068870381 @default.
• W1569200690 creator A5090953868 @default.
• W1569200690 date "1990-11-01" @default.
• W1569200690 modified "2023-09-26" @default.
• W1569200690 title "LexA-independent expression of a mutant mucAB operon" @default.
• W1569200690 cites W1482420388 @default.
• W1569200690 cites W1497428774 @default.
• W1569200690 cites W1526701908 @default.
• W1569200690 cites W1529004731 @default.
• W1569200690 cites W1529569289 @default.
• W1569200690 cites W1571486880 @default.
• W1569200690 cites W1571718539 @default.
• W1569200690 cites W1592892951 @default.
• W1569200690 cites W1618490316 @default.
• W1569200690 cites W1774448175 @default.
• W1569200690 cites W1851425750 @default.
• W1569200690 cites W1923728284 @default.
• W1569200690 cites W1952515190 @default.
• W1569200690 cites W1965267927 @default.
• W1569200690 cites W1968724116 @default.
• W1569200690 cites W1972168834 @default.
• W1569200690 cites W1974210160 @default.
• W1569200690 cites W1994203491 @default.
• W1569200690 cites W1996693838 @default.
• W1569200690 cites W2001952964 @default.
• W1569200690 cites W2015115088 @default.
• W1569200690 cites W2018289835 @default.
• W1569200690 cites W2024208506 @default.
• W1569200690 cites W2028544954 @default.
• W1569200690 cites W2029720465 @default.
• W1569200690 cites W2032118018 @default.
• W1569200690 cites W2037457710 @default.
• W1569200690 cites W2042085586 @default.
• W1569200690 cites W2056312081 @default.
• W1569200690 cites W2057180282 @default.
• W1569200690 cites W2059390535 @default.
• W1569200690 cites W2065481190 @default.
• W1569200690 cites W2065723356 @default.
• W1569200690 cites W2068270570 @default.
• W1569200690 cites W2068572804 @default.
• W1569200690 cites W2071424950 @default.
• W1569200690 cites W2088471086 @default.
• W1569200690 cites W2094983469 @default.
• W1569200690 cites W2100837269 @default.
• W1569200690 cites W2105255726 @default.
• W1569200690 cites W2118128934 @default.
• W1569200690 cites W2138270253 @default.
• W1569200690 cites W2141653906 @default.
• W1569200690 cites W2153563795 @default.
• W1569200690 cites W2318287821 @default.
• W1569200690 cites W2324164165 @default.
• W1569200690 cites W255770339 @default.
• W1569200690 cites W4248096216 @default.
• W1569200690 doi "https://doi.org/10.1128/jb.172.11.6223-6231.1990" @default.
• W1569200690 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/526803" @default.
• W1569200690 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2228957" @default.
• W1569200690 hasPublicationYear "1990" @default.
• W1569200690 type Work @default.
• W1569200690 sameAs 1569200690 @default.
• W1569200690 citedByCount "24" @default.
• W1569200690 countsByYear W15692006902012 @default.
• W1569200690 countsByYear W15692006902014 @default.
• W1569200690 countsByYear W15692006902020 @default.
• W1569200690 crossrefType "journal-article" @default.
• W1569200690 hasAuthorship W1569200690A5037692690 @default.
• W1569200690 hasAuthorship W1569200690A5068870381 @default.
• W1569200690 hasAuthorship W1569200690A5090953868 @default.
• W1569200690 hasBestOaLocation W15692006901 @default.
• W1569200690 hasConcept C104317684 @default.
• W1569200690 hasConcept C143065580 @default.
• W1569200690 hasConcept C150194340 @default.
• W1569200690 hasConcept C153911025 @default.
• W1569200690 hasConcept C158448853 @default.
• W1569200690 hasConcept C16318435 @default.
• W1569200690 hasConcept C203075996 @default.
• W1569200690 hasConcept C22744801 @default.
• W1569200690 hasConcept C2780852648 @default.
• W1569200690 hasConcept C413212 @default.
• W1569200690 hasConcept C4918238 @default.
• W1569200690 hasConcept C501734568 @default.
• W1569200690 hasConcept C54355233 @default.
• W1569200690 hasConcept C552990157 @default.
• W1569200690 hasConcept C86803240 @default.
• W1569200690 hasConceptScore W1569200690C104317684 @default.
• W1569200690 hasConceptScore W1569200690C143065580 @default.
• W1569200690 hasConceptScore W1569200690C150194340 @default.
• W1569200690 hasConceptScore W1569200690C153911025 @default.
• W1569200690 hasConceptScore W1569200690C158448853 @default.
• W1569200690 hasConceptScore W1569200690C16318435 @default.
• W1569200690 hasConceptScore W1569200690C203075996 @default.
• W1569200690 hasConceptScore W1569200690C22744801 @default.
• W1569200690 hasConceptScore W1569200690C2780852648 @default.
• W1569200690 hasConceptScore W1569200690C413212 @default.
• W1569200690 hasConceptScore W1569200690C4918238 @default.
• W1569200690 hasConceptScore W1569200690C501734568 @default.

• next