Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569212756> ?p ?o ?g. }
- W1569212756 endingPage "4196" @default.
- W1569212756 startingPage "4190" @default.
- W1569212756 abstract "Kluyveromyces lactis is a non-conventional yeast species extensively used in the expression of heterologous genes. In this study, a genetically modified K. lactis with high-level expression of α- amylase from Rhizopus oryzae was obtained, which could successfully hydrolyze and use starch for growth very well. Shake flask fermentation indicated that, the recombinant yeast was able to produce the α-amylase at considerable secretion levels using a variety of carbon sources. The highest level of amylase expression was 22.4 U/ml when cultivated at 30°C and pH 7.0 in the presence of galactose. Moreover, it was shown that the recombinant yeast, which could efficiently degrade starch, yielded a final biomass of 12.25 g/l with enzyme activity of 11 U/ml in the culture medium using 20 g soluble starch/l as the sole carbon source. Key words: Kluyveromyces lactis, α-amylase, secretion, starch, fermentation." @default.
- W1569212756 created "2016-06-24" @default.
- W1569212756 creator A5034246486 @default.
- W1569212756 creator A5040164371 @default.
- W1569212756 creator A5042938815 @default.
- W1569212756 creator A5077410212 @default.
- W1569212756 creator A5081615766 @default.
- W1569212756 date "2011-01-01" @default.
- W1569212756 modified "2023-09-23" @default.
- W1569212756 title "Secretory expression of Rhizopus oryzae α-amylase in Kluyveromyces lactis" @default.
- W1569212756 cites W1533886852 @default.
- W1569212756 cites W1926121210 @default.
- W1569212756 cites W1966295465 @default.
- W1569212756 cites W1968206838 @default.
- W1569212756 cites W1985108159 @default.
- W1569212756 cites W1988657972 @default.
- W1569212756 cites W1989880904 @default.
- W1569212756 cites W1990398418 @default.
- W1569212756 cites W2009674410 @default.
- W1569212756 cites W2011279180 @default.
- W1569212756 cites W2011591693 @default.
- W1569212756 cites W2012063005 @default.
- W1569212756 cites W2017186389 @default.
- W1569212756 cites W2036675705 @default.
- W1569212756 cites W2046689037 @default.
- W1569212756 cites W2047597963 @default.
- W1569212756 cites W207500929 @default.
- W1569212756 cites W2078373711 @default.
- W1569212756 cites W2082579794 @default.
- W1569212756 cites W2092846883 @default.
- W1569212756 cites W2097588241 @default.
- W1569212756 cites W2100513481 @default.
- W1569212756 cites W2138927187 @default.
- W1569212756 cites W2149566215 @default.
- W1569212756 cites W2167665128 @default.
- W1569212756 cites W2170241752 @default.
- W1569212756 cites W2296999543 @default.
- W1569212756 hasPublicationYear "2011" @default.
- W1569212756 type Work @default.
- W1569212756 sameAs 1569212756 @default.
- W1569212756 citedByCount "1" @default.
- W1569212756 countsByYear W15692127562013 @default.
- W1569212756 crossrefType "journal-article" @default.
- W1569212756 hasAuthorship W1569212756A5034246486 @default.
- W1569212756 hasAuthorship W1569212756A5040164371 @default.
- W1569212756 hasAuthorship W1569212756A5042938815 @default.
- W1569212756 hasAuthorship W1569212756A5077410212 @default.
- W1569212756 hasAuthorship W1569212756A5081615766 @default.
- W1569212756 hasConcept C100544194 @default.
- W1569212756 hasConcept C104317684 @default.
- W1569212756 hasConcept C108305142 @default.
- W1569212756 hasConcept C170835558 @default.
- W1569212756 hasConcept C181199279 @default.
- W1569212756 hasConcept C185592680 @default.
- W1569212756 hasConcept C2776917750 @default.
- W1569212756 hasConcept C2777576037 @default.
- W1569212756 hasConcept C2778041695 @default.
- W1569212756 hasConcept C2778197599 @default.
- W1569212756 hasConcept C2778541762 @default.
- W1569212756 hasConcept C2778753027 @default.
- W1569212756 hasConcept C2779222958 @default.
- W1569212756 hasConcept C2780115692 @default.
- W1569212756 hasConcept C2780764520 @default.
- W1569212756 hasConcept C2781204770 @default.
- W1569212756 hasConcept C31903555 @default.
- W1569212756 hasConcept C40767141 @default.
- W1569212756 hasConcept C529335014 @default.
- W1569212756 hasConcept C55493867 @default.
- W1569212756 hasConcept C86803240 @default.
- W1569212756 hasConcept C89423630 @default.
- W1569212756 hasConceptScore W1569212756C100544194 @default.
- W1569212756 hasConceptScore W1569212756C104317684 @default.
- W1569212756 hasConceptScore W1569212756C108305142 @default.
- W1569212756 hasConceptScore W1569212756C170835558 @default.
- W1569212756 hasConceptScore W1569212756C181199279 @default.
- W1569212756 hasConceptScore W1569212756C185592680 @default.
- W1569212756 hasConceptScore W1569212756C2776917750 @default.
- W1569212756 hasConceptScore W1569212756C2777576037 @default.
- W1569212756 hasConceptScore W1569212756C2778041695 @default.
- W1569212756 hasConceptScore W1569212756C2778197599 @default.
- W1569212756 hasConceptScore W1569212756C2778541762 @default.
- W1569212756 hasConceptScore W1569212756C2778753027 @default.
- W1569212756 hasConceptScore W1569212756C2779222958 @default.
- W1569212756 hasConceptScore W1569212756C2780115692 @default.
- W1569212756 hasConceptScore W1569212756C2780764520 @default.
- W1569212756 hasConceptScore W1569212756C2781204770 @default.
- W1569212756 hasConceptScore W1569212756C31903555 @default.
- W1569212756 hasConceptScore W1569212756C40767141 @default.
- W1569212756 hasConceptScore W1569212756C529335014 @default.
- W1569212756 hasConceptScore W1569212756C55493867 @default.
- W1569212756 hasConceptScore W1569212756C86803240 @default.
- W1569212756 hasConceptScore W1569212756C89423630 @default.
- W1569212756 hasIssue "20" @default.
- W1569212756 hasLocation W15692127561 @default.
- W1569212756 hasOpenAccess W1569212756 @default.
- W1569212756 hasPrimaryLocation W15692127561 @default.
- W1569212756 hasRelatedWork W102114844 @default.
- W1569212756 hasRelatedWork W1486218956 @default.