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• W1569224274 startingPage "11528" @default.
• W1569224274 abstract "Avian retroviral DNA is rapidly ligated and transcribed following microinjection into chick cells. To analyze the fidelity of intranuclear ligations, DNA molecules with a variety of protruding ends or flush ends were co-injected into env-deficient Rous sarcoma virus-infected (RSV(-] cells. Two fragments were chosen which could complement the RSV(-) env deficiency upon ligation. This sensitive biological assay indicated that intracellular ligation between molecules with complementary protruding ends or flush ends was relatively efficient. Ligation was not observed with non-complementary termini. Furthermore, when multiple DNA molecules possessing a variety of protruding termini were introduced into the cells, complementary termini were selectively joined. Finally, when viral DNAs which expressed separate genetic markers were co-injected, almost 50% of the virus subsequently released were recombinants, indicating that ligations generally involved multiple molecules." @default.
• W1569224274 created "2016-06-24" @default.
• W1569224274 creator A5015090263 @default.
• W1569224274 creator A5054006122 @default.
• W1569224274 date "1983-10-01" @default.
• W1569224274 modified "2023-09-27" @default.
• W1569224274 title "Selective ligation of DNA molecules following microinjection." @default.
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• W1569224274 doi "https://doi.org/10.1016/s0021-9258(17)44260-8" @default.
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