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- W1569233644 abstract "Summary The preparation of tumor ribosomes that are essentially free of polyribosomes and active in polypeptide synthesis is described. Most active preparations were obtained by incubating ribosomal suspensions 20 min under conditions of protein synthesis. After this time, all polysomes had been converted to 80 S particles which could be stimulated to polypeptide synthesis by polyuridylic acid or liver mRNA. High-speed supernatant from rat or pig liver was used for the incubation procedure and in the cell-free system, rather than tumor supernatant, which caused almost complete inactivation of the ribosomes. The most active high-energy system was 10 mm 3-phosphoglycerate; optimum ATP and Mg2+ concentrations were 1 and 7 mm, respectively. Deoxycholate was used to solubilize membranous fractions and to liberate bound polysomes. Ribosomes, having been prepared by deoxycholate treatment and successive incubation, were free of polyribosomes. However, a small amount of ribosomal subunits was always observed under these conditions. Large-scale preparation of structurally intact subunits by Mg2+ deficiency or mild ethylenediaminetetra-acetic acid treatment is complicated by the high affinity between 40 and 60 S particles. In this respect, ribosomes of the Ehrlich carcinoma are comparable to those of rapidly growing hepatomas. However, they are strikingly different from ribosomes of slowly growing hepatomas and normal tissues." @default.
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- W1569233644 date "1972-12-01" @default.
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- W1569233644 title "Preparation and characterization of 80 S ribosomes of ascites tumor cells active in polypeptide synthesis." @default.
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