FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569269505> ?p ?o ?g. }

• W1569269505 abstract "Transcript quantification methodology has evolved from Northern blots to RNase protection to competitive RT-PCR. Each of these methods made strides toward greater sensitivity and accuracy in quantification. Since the introduction of the 7700 Sequence Detection System and Taqman® chemistry by Applied Biosystems in 1996, quantitative real-time PCR has become widely accepted as the most sensitive and accurate method for the quantification of both RNA and DNA from a wide variety of sources. Today, there are a number of real-time instruments from a variety of vendors and several detection methodologies and kit chemistries available. At the same time, prices for real-time platforms are falling dramatically. This trend means that the technology is moving from one found primarily in core laboratories to the bench tops of individual investigators. There are several ways to interrogate a cell for changes induced by artificial or natural agents during a biological process. One way is to look for changes in cellular transcript levels that may indicate downstream changes in the corresponding protein. In another instance, the focus may be on the presence or absence of a viral or bacterial pathogen. In this case, detecting not only the presence but also the degree of infection provides valuable information. Alternatively, looking for the presence or the level of expression from a transgene or the inhibition of expression of an endogenous gene by an RNAi agent may be the question of interest. In all cases, quantitative real-time PCR technology can be utilized to provide the required results. However, successful implementation of the technology requires the user to have a basic background in the theoretical principles of real-time PCR as well as their practical application. The goal of this review is to provide this information. It will not be possible to cover all aspects of real-time PCR in this review. For a more comprehensive overview of real-time PCR, “A-Z of Quantitative PCR,” edited by Stephen Bustin, is highly recommended." @default.
• W1569269505 created "2016-06-24" @default.
• W1569269505 creator A5054312539 @default.
• W1569269505 date "2006-09-15" @default.
• W1569269505 modified "2023-09-23" @default.
• W1569269505 title "Real-Time Quantitative PCR: Theory and Practice" @default.
• W1569269505 cites W1573011590 @default.
• W1569269505 cites W1577577364 @default.
• W1569269505 cites W1599075286 @default.
• W1569269505 cites W1973708871 @default.
• W1569269505 cites W1980848219 @default.
• W1569269505 cites W1981228431 @default.
• W1569269505 cites W1981497491 @default.
• W1569269505 cites W1988462426 @default.
• W1569269505 cites W1996880521 @default.
• W1569269505 cites W2000349847 @default.
• W1569269505 cites W2026654076 @default.
• W1569269505 cites W2044478281 @default.
• W1569269505 cites W2057974580 @default.
• W1569269505 cites W2058327969 @default.
• W1569269505 cites W2062376685 @default.
• W1569269505 cites W2093009120 @default.
• W1569269505 cites W2099300333 @default.
• W1569269505 cites W2106700932 @default.
• W1569269505 cites W2112475505 @default.
• W1569269505 cites W2112982595 @default.
• W1569269505 cites W2117483311 @default.
• W1569269505 cites W2121396784 @default.
• W1569269505 cites W2122556781 @default.
• W1569269505 cites W2140451732 @default.
• W1569269505 cites W2142722173 @default.
• W1569269505 cites W2143146123 @default.
• W1569269505 cites W2145684092 @default.
• W1569269505 cites W2148797402 @default.
• W1569269505 cites W2149551170 @default.
• W1569269505 cites W2149715896 @default.
• W1569269505 cites W2153022294 @default.
• W1569269505 cites W2153847854 @default.
• W1569269505 cites W2164578725 @default.
• W1569269505 cites W2285277823 @default.
• W1569269505 cites W2334397963 @default.
• W1569269505 cites W4230732007 @default.
• W1569269505 cites W4239629847 @default.
• W1569269505 doi "https://doi.org/10.1002/3527600906.mcb.200500012" @default.
• W1569269505 hasPublicationYear "2006" @default.
• W1569269505 type Work @default.
• W1569269505 sameAs 1569269505 @default.
• W1569269505 citedByCount "0" @default.
• W1569269505 crossrefType "reference-entry" @default.
• W1569269505 hasAuthorship W1569269505A5054312539 @default.
• W1569269505 hasConcept C104317684 @default.
• W1569269505 hasConcept C143660080 @default.
• W1569269505 hasConcept C166703698 @default.
• W1569269505 hasConcept C48023723 @default.
• W1569269505 hasConcept C54355233 @default.
• W1569269505 hasConcept C67705224 @default.
• W1569269505 hasConcept C70721500 @default.
• W1569269505 hasConcept C86803240 @default.
• W1569269505 hasConceptScore W1569269505C104317684 @default.
• W1569269505 hasConceptScore W1569269505C143660080 @default.
• W1569269505 hasConceptScore W1569269505C166703698 @default.
• W1569269505 hasConceptScore W1569269505C48023723 @default.
• W1569269505 hasConceptScore W1569269505C54355233 @default.
• W1569269505 hasConceptScore W1569269505C67705224 @default.
• W1569269505 hasConceptScore W1569269505C70721500 @default.
• W1569269505 hasConceptScore W1569269505C86803240 @default.
• W1569269505 hasLocation W15692695051 @default.
• W1569269505 hasOpenAccess W1569269505 @default.
• W1569269505 hasPrimaryLocation W15692695051 @default.
• W1569269505 hasRelatedWork W19166555 @default.
• W1569269505 hasRelatedWork W2016840328 @default.
• W1569269505 hasRelatedWork W2024508004 @default.
• W1569269505 hasRelatedWork W2041332439 @default.
• W1569269505 hasRelatedWork W2128088040 @default.
• W1569269505 hasRelatedWork W2128165354 @default.
• W1569269505 hasRelatedWork W2168712887 @default.
• W1569269505 hasRelatedWork W2404074437 @default.
• W1569269505 hasRelatedWork W4312030497 @default.
• W1569269505 hasRelatedWork W2741827410 @default.
• W1569269505 isParatext "false" @default.
• W1569269505 isRetracted "false" @default.
• W1569269505 magId "1569269505" @default.
• W1569269505 workType "reference-entry" @default.