Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569276900> ?p ?o ?g. }
- W1569276900 endingPage "352" @default.
- W1569276900 startingPage "347" @default.
- W1569276900 abstract "Comparisons of 16S rDNA sequences were used to determine the phylogeny of not-yet-cultured spirochetes from hindguts of the African higher termite, Nasutitermes lujae (Wasmann). The 16S rRNA genes were amplified directly from spirochete-rich hindguts by using universal primers, and the amplified products were cloned into Escherichia coli. Clones were screened with a spirochete-specific DNA probe. Analysis of 1,410 base positions of the 16S rDNA insert from one spirochete clone, designated NL1, supported its assignment to the genus Treponema, with average interspecies similarities of ca. 85%. The sequence of NL1 was most closely related (ca. 87 to 88% similarity) to sequences of Spirochaeta stenostrepta and Spirochaeta caldaria and to a previously published sequence (ca. 87% similarity) of spirochetal clone MDS1 from the Australian lower termite, Mastotermes darwiniensis (Froggatt). On the basis of 16S rRNA sequence comparisons and individual base signatures, clones NL1 and MDS1 clearly represent two novel species of Treponema, although specific epithets have not yet been proposed. The gross morphology of NL1 was determined from in situ hybridization experiments with an NL1-specific, fluorescently labeled oligonucleotide probe. Cells were approximately 0.3 to 0.4 by 30 microns in size, with a wavelength and amplitude of about 10 microns and 0.8 to 1.6 micron, respectively. Moreover, electron microscopy of various undulate cells present in gut contents confirmed that they possessed ultrastructural features typical of spirochetes, i.e., a wavy protoplasmic cylinder, periplasmic flagella, and an outer sheath. The sequence data suggest that termite gut spirochetes may represent a separate line of descent from other treponemes and that they constitute a significant reservoir of previously unrecognized spirochetal biodiversity." @default.
- W1569276900 created "2016-06-24" @default.
- W1569276900 creator A5009509854 @default.
- W1569276900 creator A5037667753 @default.
- W1569276900 creator A5039151831 @default.
- W1569276900 creator A5064446453 @default.
- W1569276900 creator A5069863581 @default.
- W1569276900 creator A5085422017 @default.
- W1569276900 date "1996-02-01" @default.
- W1569276900 modified "2023-09-25" @default.
- W1569276900 title "Phylogeny of not-yet-cultured spirochetes from termite guts" @default.
- W1569276900 cites W1484352718 @default.
- W1569276900 cites W1525734744 @default.
- W1569276900 cites W1535624277 @default.
- W1569276900 cites W1561580658 @default.
- W1569276900 cites W1573139894 @default.
- W1569276900 cites W1574952313 @default.
- W1569276900 cites W1605925025 @default.
- W1569276900 cites W1829469363 @default.
- W1569276900 cites W1848498826 @default.
- W1569276900 cites W1911123181 @default.
- W1569276900 cites W1966227798 @default.
- W1569276900 cites W1974590216 @default.
- W1569276900 cites W2019151491 @default.
- W1569276900 cites W2052639391 @default.
- W1569276900 cites W2054031439 @default.
- W1569276900 cites W2059378496 @default.
- W1569276900 cites W2059462592 @default.
- W1569276900 cites W2067665219 @default.
- W1569276900 cites W2096507832 @default.
- W1569276900 cites W2097706568 @default.
- W1569276900 cites W2104412777 @default.
- W1569276900 cites W2105210691 @default.
- W1569276900 cites W2106274663 @default.
- W1569276900 cites W2128113743 @default.
- W1569276900 cites W2131988453 @default.
- W1569276900 cites W2154401636 @default.
- W1569276900 cites W2158459091 @default.
- W1569276900 cites W2166865790 @default.
- W1569276900 cites W2177709753 @default.
- W1569276900 cites W346827628 @default.
- W1569276900 cites W41165409 @default.
- W1569276900 cites W3184243149 @default.
- W1569276900 doi "https://doi.org/10.1128/aem.62.2.347-352.1996" @default.
- W1569276900 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/167805" @default.
- W1569276900 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8593040" @default.
- W1569276900 hasPublicationYear "1996" @default.
- W1569276900 type Work @default.
- W1569276900 sameAs 1569276900 @default.
- W1569276900 citedByCount "101" @default.
- W1569276900 countsByYear W15692769002012 @default.
- W1569276900 countsByYear W15692769002013 @default.
- W1569276900 countsByYear W15692769002015 @default.
- W1569276900 countsByYear W15692769002018 @default.
- W1569276900 countsByYear W15692769002019 @default.
- W1569276900 countsByYear W15692769002020 @default.
- W1569276900 countsByYear W15692769002021 @default.
- W1569276900 countsByYear W15692769002022 @default.
- W1569276900 countsByYear W15692769002023 @default.
- W1569276900 crossrefType "journal-article" @default.
- W1569276900 hasAuthorship W1569276900A5009509854 @default.
- W1569276900 hasAuthorship W1569276900A5037667753 @default.
- W1569276900 hasAuthorship W1569276900A5039151831 @default.
- W1569276900 hasAuthorship W1569276900A5064446453 @default.
- W1569276900 hasAuthorship W1569276900A5069863581 @default.
- W1569276900 hasAuthorship W1569276900A5085422017 @default.
- W1569276900 hasBestOaLocation W15692769001 @default.
- W1569276900 hasConcept C104317684 @default.
- W1569276900 hasConcept C111824103 @default.
- W1569276900 hasConcept C129312508 @default.
- W1569276900 hasConcept C153911025 @default.
- W1569276900 hasConcept C159047783 @default.
- W1569276900 hasConcept C207812311 @default.
- W1569276900 hasConcept C2776983459 @default.
- W1569276900 hasConcept C2780187896 @default.
- W1569276900 hasConcept C3013748606 @default.
- W1569276900 hasConcept C42062724 @default.
- W1569276900 hasConcept C523546767 @default.
- W1569276900 hasConcept C54355233 @default.
- W1569276900 hasConcept C81089528 @default.
- W1569276900 hasConcept C86803240 @default.
- W1569276900 hasConcept C89423630 @default.
- W1569276900 hasConcept C90132467 @default.
- W1569276900 hasConceptScore W1569276900C104317684 @default.
- W1569276900 hasConceptScore W1569276900C111824103 @default.
- W1569276900 hasConceptScore W1569276900C129312508 @default.
- W1569276900 hasConceptScore W1569276900C153911025 @default.
- W1569276900 hasConceptScore W1569276900C159047783 @default.
- W1569276900 hasConceptScore W1569276900C207812311 @default.
- W1569276900 hasConceptScore W1569276900C2776983459 @default.
- W1569276900 hasConceptScore W1569276900C2780187896 @default.
- W1569276900 hasConceptScore W1569276900C3013748606 @default.
- W1569276900 hasConceptScore W1569276900C42062724 @default.
- W1569276900 hasConceptScore W1569276900C523546767 @default.
- W1569276900 hasConceptScore W1569276900C54355233 @default.
- W1569276900 hasConceptScore W1569276900C81089528 @default.
- W1569276900 hasConceptScore W1569276900C86803240 @default.
- W1569276900 hasConceptScore W1569276900C89423630 @default.