Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569320589> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1569320589 abstract "Cell-free circulating (CFC) DNA is now considered an important biomarker for early detection of cancer, residual disease, monitoring chemotherapy and other aspects of cancer management. The isolation of CFC-DNA from plasma as a liquid biopsy may begin replacing more invasive tissue biopsies as a means to detect and analyze cancer mutations. Unfortunately, conventional techniques for the isolation of CFC-DNA from plasma require a relatively time consuming and complex process which would rule out their use for point of care diagnostic applications. This work demonstrated the rapid isolation and detection of both single-stranded and double-stranded HMW-DNA and 40 nm nanoparticles directly from whole blood and buffy coat blood samples. We then went on to show the rapid isolation and detection of SYBR Green stained CFC- DNA from 20 [mu]L whole blood samples from Chronic Lymphocytic Leukemia (CLL) patients, as well as isolation of cell-free mitochondria and virus from high conductivity buffers. To investigate the potentially clinical utility of this technology, we isolated CFC-DNA from fresh blood samples of fifteen CLL patients and three healthy individuals. CFC-DNA from 25 [mu]L (a drop) of blood was separated and concentrated into DEP high-field regions in about three minutes and held while blood cells, proteins and other biomolecules were removed by a fluidic wash. Concentrated CFC-DNA was detected by fluorescence and then eluted for PCR and DNA sequencing. The complete process, blood to CFC-DNA for PCR, spanned less than 10 minutes. Eluted CFC-DNA, from 5 [mu]l of the original CLL blood sample, was amplified by PCR using IGVH-specific primers to identify the unique IGHV gene expressed by the leukemic B-cell clone. The PCR results obtained by DEP from CLL blood were comparable to results obtained using conventional sample preparation of CFC-DNA starting with one ml of plasma and the sequencing results were accurate for all 15 patient samples. The ability of DEP to provide rapid isolation of CFC-DNA from the equivalent of a drop of blood represents a major step forward in the mission to create viable point of care cancer diagnostics and patient monitoring. This technology is applicable to a wide variety of biomarkers in biological and environmental samples and has broad implications as low-complexity and easily integrated front-end sample processing solution" @default.
- W1569320589 created "2016-06-24" @default.
- W1569320589 creator A5038666359 @default.
- W1569320589 date "2014-12-20" @default.
- W1569320589 modified "2023-09-27" @default.
- W1569320589 title "Dielectrophoresis for biomarker isolation from biological samples" @default.
- W1569320589 hasPublicationYear "2014" @default.
- W1569320589 type Work @default.
- W1569320589 sameAs 1569320589 @default.
- W1569320589 citedByCount "0" @default.
- W1569320589 crossrefType "journal-article" @default.
- W1569320589 hasAuthorship W1569320589A5038666359 @default.
- W1569320589 hasConcept C104317684 @default.
- W1569320589 hasConcept C121608353 @default.
- W1569320589 hasConcept C152110520 @default.
- W1569320589 hasConcept C153911025 @default.
- W1569320589 hasConcept C1621761 @default.
- W1569320589 hasConcept C172680121 @default.
- W1569320589 hasConcept C203014093 @default.
- W1569320589 hasConcept C2775941552 @default.
- W1569320589 hasConcept C2778258057 @default.
- W1569320589 hasConcept C2778285947 @default.
- W1569320589 hasConcept C2778461978 @default.
- W1569320589 hasConcept C2779234561 @default.
- W1569320589 hasConcept C2779529041 @default.
- W1569320589 hasConcept C2779823535 @default.
- W1569320589 hasConcept C48126324 @default.
- W1569320589 hasConcept C49105822 @default.
- W1569320589 hasConcept C54355233 @default.
- W1569320589 hasConcept C552990157 @default.
- W1569320589 hasConcept C55493867 @default.
- W1569320589 hasConcept C60644358 @default.
- W1569320589 hasConcept C78063203 @default.
- W1569320589 hasConcept C86803240 @default.
- W1569320589 hasConceptScore W1569320589C104317684 @default.
- W1569320589 hasConceptScore W1569320589C121608353 @default.
- W1569320589 hasConceptScore W1569320589C152110520 @default.
- W1569320589 hasConceptScore W1569320589C153911025 @default.
- W1569320589 hasConceptScore W1569320589C1621761 @default.
- W1569320589 hasConceptScore W1569320589C172680121 @default.
- W1569320589 hasConceptScore W1569320589C203014093 @default.
- W1569320589 hasConceptScore W1569320589C2775941552 @default.
- W1569320589 hasConceptScore W1569320589C2778258057 @default.
- W1569320589 hasConceptScore W1569320589C2778285947 @default.
- W1569320589 hasConceptScore W1569320589C2778461978 @default.
- W1569320589 hasConceptScore W1569320589C2779234561 @default.
- W1569320589 hasConceptScore W1569320589C2779529041 @default.
- W1569320589 hasConceptScore W1569320589C2779823535 @default.
- W1569320589 hasConceptScore W1569320589C48126324 @default.
- W1569320589 hasConceptScore W1569320589C49105822 @default.
- W1569320589 hasConceptScore W1569320589C54355233 @default.
- W1569320589 hasConceptScore W1569320589C552990157 @default.
- W1569320589 hasConceptScore W1569320589C55493867 @default.
- W1569320589 hasConceptScore W1569320589C60644358 @default.
- W1569320589 hasConceptScore W1569320589C78063203 @default.
- W1569320589 hasConceptScore W1569320589C86803240 @default.
- W1569320589 hasLocation W15693205891 @default.
- W1569320589 hasOpenAccess W1569320589 @default.
- W1569320589 hasPrimaryLocation W15693205891 @default.
- W1569320589 hasRelatedWork W100711220 @default.
- W1569320589 hasRelatedWork W1964182026 @default.
- W1569320589 hasRelatedWork W1974179026 @default.
- W1569320589 hasRelatedWork W1997521518 @default.
- W1569320589 hasRelatedWork W2009419250 @default.
- W1569320589 hasRelatedWork W2010187210 @default.
- W1569320589 hasRelatedWork W2060633466 @default.
- W1569320589 hasRelatedWork W2099825978 @default.
- W1569320589 hasRelatedWork W2130478804 @default.
- W1569320589 hasRelatedWork W2146193470 @default.
- W1569320589 hasRelatedWork W2155245333 @default.
- W1569320589 hasRelatedWork W2291844600 @default.
- W1569320589 hasRelatedWork W2323171068 @default.
- W1569320589 hasRelatedWork W2602958922 @default.
- W1569320589 hasRelatedWork W2788759450 @default.
- W1569320589 hasRelatedWork W2789965031 @default.
- W1569320589 hasRelatedWork W2886495745 @default.
- W1569320589 hasRelatedWork W2952755989 @default.
- W1569320589 hasRelatedWork W3044768128 @default.
- W1569320589 hasRelatedWork W2782222382 @default.
- W1569320589 isParatext "false" @default.
- W1569320589 isRetracted "false" @default.
- W1569320589 magId "1569320589" @default.
- W1569320589 workType "article" @default.