Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569359424> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1569359424 endingPage "386" @default.
- W1569359424 startingPage "377" @default.
- W1569359424 abstract "Arginine decarboxylase which makes its appearance in Lathyrus sativus seedlings after 24 h of seed germination reaches its highest level around 5-7 days, the cotyledons containing about 60% of the total activity in the seedlings at day 5. The cytosol enzyme was purified 977-fold from whole seedlings by steps involving manganese chloride treatment, ammonium sulphate and acetone fractionations, positive adsorption on alumina C-gamma gel, DEAE-Sephadex chromatography followed by preparative disc gel electrophoresis. The enzyme was shown to be homogeneous by electrophoretic and immunological criteria, had a molecular weight of 220,000 and appears to be a hexamer with identical subunits. The optimal pH and temperature for the enzyme activity were 8.5 and 45 degrees C respectively. The enzyme follows typical Michaelis-Menten kinetics with a Km value of 1.73 mM for arginine. Though Mn2+ at lower concentrations stimulated the enzyme activity, there was no dependence of the enzyme on any metal for the activity. The arginine decarboxylase of L. sativus is a sulfhydryl enzyme. The data on co-factor requirement, inhibition by carbonyl reagents, reducing agents and pyridoxal phosphate inhibitors, and a partial reversal by pyridoxal phosphate of inhibition by pyridoxal-HCl suggests that pyridoxal 5'-phosphate is involved as a co-factor for the enzyme. The enzyme activity was inhibited competitively by various amines including the product agmatine. Highest inhibition was obtained with spermine and arcain. The substrate analogue, L-canavanine, homologue L-homoarginine and other basic amino acids like L-lysine and L-ornithine inhibited the enzyme activity competitively, homoarginine being the most effective in this respect." @default.
- W1569359424 created "2016-06-24" @default.
- W1569359424 creator A5025836020 @default.
- W1569359424 creator A5051525443 @default.
- W1569359424 date "1975-11-01" @default.
- W1569359424 modified "2023-10-18" @default.
- W1569359424 title "Arginine Decarboxylase from <i>Lathyrus sativus</i> Seedlings" @default.
- W1569359424 cites W1413886773 @default.
- W1569359424 cites W1480225220 @default.
- W1569359424 cites W1501927495 @default.
- W1569359424 cites W150363975 @default.
- W1569359424 cites W1541698544 @default.
- W1569359424 cites W1575372103 @default.
- W1569359424 cites W1578349954 @default.
- W1569359424 cites W1592484600 @default.
- W1569359424 cites W1606964978 @default.
- W1569359424 cites W1625660792 @default.
- W1569359424 cites W1650378596 @default.
- W1569359424 cites W1750773183 @default.
- W1569359424 cites W1775749144 @default.
- W1569359424 cites W1976317042 @default.
- W1569359424 cites W1996741416 @default.
- W1569359424 cites W2012102268 @default.
- W1569359424 cites W2012325572 @default.
- W1569359424 cites W2019210455 @default.
- W1569359424 cites W2024448878 @default.
- W1569359424 cites W2029869847 @default.
- W1569359424 cites W2035444631 @default.
- W1569359424 cites W2066839442 @default.
- W1569359424 cites W2073156258 @default.
- W1569359424 cites W2111301002 @default.
- W1569359424 doi "https://doi.org/10.1111/j.1432-1033.1975.tb02465.x" @default.
- W1569359424 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1252" @default.
- W1569359424 hasPublicationYear "1975" @default.
- W1569359424 type Work @default.
- W1569359424 sameAs 1569359424 @default.
- W1569359424 citedByCount "59" @default.
- W1569359424 countsByYear W15693594242012 @default.
- W1569359424 countsByYear W15693594242013 @default.
- W1569359424 countsByYear W15693594242015 @default.
- W1569359424 countsByYear W15693594242019 @default.
- W1569359424 countsByYear W15693594242020 @default.
- W1569359424 countsByYear W15693594242023 @default.
- W1569359424 crossrefType "journal-article" @default.
- W1569359424 hasAuthorship W1569359424A5025836020 @default.
- W1569359424 hasAuthorship W1569359424A5051525443 @default.
- W1569359424 hasBestOaLocation W15693594241 @default.
- W1569359424 hasConcept C181199279 @default.
- W1569359424 hasConcept C185592680 @default.
- W1569359424 hasConcept C197957613 @default.
- W1569359424 hasConcept C2776834122 @default.
- W1569359424 hasConcept C2777107663 @default.
- W1569359424 hasConcept C2777167505 @default.
- W1569359424 hasConcept C2777468819 @default.
- W1569359424 hasConcept C2780859938 @default.
- W1569359424 hasConcept C28212737 @default.
- W1569359424 hasConcept C43617362 @default.
- W1569359424 hasConcept C515207424 @default.
- W1569359424 hasConcept C55493867 @default.
- W1569359424 hasConceptScore W1569359424C181199279 @default.
- W1569359424 hasConceptScore W1569359424C185592680 @default.
- W1569359424 hasConceptScore W1569359424C197957613 @default.
- W1569359424 hasConceptScore W1569359424C2776834122 @default.
- W1569359424 hasConceptScore W1569359424C2777107663 @default.
- W1569359424 hasConceptScore W1569359424C2777167505 @default.
- W1569359424 hasConceptScore W1569359424C2777468819 @default.
- W1569359424 hasConceptScore W1569359424C2780859938 @default.
- W1569359424 hasConceptScore W1569359424C28212737 @default.
- W1569359424 hasConceptScore W1569359424C43617362 @default.
- W1569359424 hasConceptScore W1569359424C515207424 @default.
- W1569359424 hasConceptScore W1569359424C55493867 @default.
- W1569359424 hasIssue "2" @default.
- W1569359424 hasLocation W15693594241 @default.
- W1569359424 hasLocation W15693594242 @default.
- W1569359424 hasOpenAccess W1569359424 @default.
- W1569359424 hasPrimaryLocation W15693594241 @default.
- W1569359424 hasRelatedWork W1543940870 @default.
- W1569359424 hasRelatedWork W190039173 @default.
- W1569359424 hasRelatedWork W2376104818 @default.
- W1569359424 hasRelatedWork W2442283683 @default.
- W1569359424 hasRelatedWork W2555274242 @default.
- W1569359424 hasRelatedWork W3007640735 @default.
- W1569359424 hasRelatedWork W3163829211 @default.
- W1569359424 hasRelatedWork W3176538296 @default.
- W1569359424 hasRelatedWork W2334306123 @default.
- W1569359424 hasRelatedWork W2518616098 @default.
- W1569359424 hasVolume "59" @default.
- W1569359424 isParatext "false" @default.
- W1569359424 isRetracted "false" @default.
- W1569359424 magId "1569359424" @default.
- W1569359424 workType "article" @default.