Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569365736> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1569365736 endingPage "1848" @default.
- W1569365736 startingPage "1843" @default.
- W1569365736 abstract "Abstract The tumor necrosis factor (TNF) promoter and 3'-untranslated region (3'-UTR) each contain sequence elements that mediate a response to bacterial endotoxin. Although the promoter contains sequences that permit augmented TNF gene transcription in response to LPS, the 3'-UTR contains sequences that normally confer translational repression, but which allow derepression to occur after cell contact with endotoxin. We now show that both genetic elements act in concert during activation of TNF gene expression in macrophages. In order to do so, we have made use of chloramphenicol acetyltransferase reporter constructs in which the TNF promoter and 3'-UTR are represented either independently or in combination with one another. Suppression of chloramphenicol acetyltransferase and TNF mRNA synthesis, observed after treatment of the macrophages with dexamethasone, 2-aminopurine, pentoxifylline, or dibutyryl cAMP, has also been studied in detail. Each class of inhibitor suppresses TNF biosynthesis through a separate mechanism. Interestingly, suppression by pentoxifylline is manifested partly (but not entirely) at the level of transcription, and depends upon the presence of both the TNF promoter and 3'-UTR. The data suggest that other sequences within the TNF gene could also be required for the full effect of pentoxifylline, which may act to prevent processing of the primary transcript. The suppressive effect of dexamethasone is manifested both at the level of transcription and at the level of translation, and is mediated both by sequences present in the TNF promoter and by sequences present in the 3'-UTR. Suppression by 2-aminopurine is solely dependent upon promoter sequences." @default.
- W1569365736 created "2016-06-24" @default.
- W1569365736 creator A5001203307 @default.
- W1569365736 creator A5055634803 @default.
- W1569365736 creator A5059140393 @default.
- W1569365736 date "1991-03-15" @default.
- W1569365736 modified "2023-10-17" @default.
- W1569365736 title "Interactive effects of the tumor necrosis factor promoter and 3'-untranslated regions." @default.
- W1569365736 doi "https://doi.org/10.4049/jimmunol.146.6.1843" @default.
- W1569365736 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1848573" @default.
- W1569365736 hasPublicationYear "1991" @default.
- W1569365736 type Work @default.
- W1569365736 sameAs 1569365736 @default.
- W1569365736 citedByCount "115" @default.
- W1569365736 countsByYear W15693657362014 @default.
- W1569365736 countsByYear W15693657362016 @default.
- W1569365736 countsByYear W15693657362018 @default.
- W1569365736 countsByYear W15693657362022 @default.
- W1569365736 countsByYear W15693657362023 @default.
- W1569365736 crossrefType "journal-article" @default.
- W1569365736 hasAuthorship W1569365736A5001203307 @default.
- W1569365736 hasAuthorship W1569365736A5055634803 @default.
- W1569365736 hasAuthorship W1569365736A5059140393 @default.
- W1569365736 hasBestOaLocation W15693657361 @default.
- W1569365736 hasConcept C101762097 @default.
- W1569365736 hasConcept C104317684 @default.
- W1569365736 hasConcept C105580179 @default.
- W1569365736 hasConcept C138885662 @default.
- W1569365736 hasConcept C150194340 @default.
- W1569365736 hasConcept C153911025 @default.
- W1569365736 hasConcept C161733203 @default.
- W1569365736 hasConcept C179926584 @default.
- W1569365736 hasConcept C186310378 @default.
- W1569365736 hasConcept C22667442 @default.
- W1569365736 hasConcept C2780404050 @default.
- W1569365736 hasConcept C2780730277 @default.
- W1569365736 hasConcept C41895202 @default.
- W1569365736 hasConcept C54355233 @default.
- W1569365736 hasConcept C73766848 @default.
- W1569365736 hasConcept C86339819 @default.
- W1569365736 hasConcept C86803240 @default.
- W1569365736 hasConcept C89604277 @default.
- W1569365736 hasConcept C98274493 @default.
- W1569365736 hasConceptScore W1569365736C101762097 @default.
- W1569365736 hasConceptScore W1569365736C104317684 @default.
- W1569365736 hasConceptScore W1569365736C105580179 @default.
- W1569365736 hasConceptScore W1569365736C138885662 @default.
- W1569365736 hasConceptScore W1569365736C150194340 @default.
- W1569365736 hasConceptScore W1569365736C153911025 @default.
- W1569365736 hasConceptScore W1569365736C161733203 @default.
- W1569365736 hasConceptScore W1569365736C179926584 @default.
- W1569365736 hasConceptScore W1569365736C186310378 @default.
- W1569365736 hasConceptScore W1569365736C22667442 @default.
- W1569365736 hasConceptScore W1569365736C2780404050 @default.
- W1569365736 hasConceptScore W1569365736C2780730277 @default.
- W1569365736 hasConceptScore W1569365736C41895202 @default.
- W1569365736 hasConceptScore W1569365736C54355233 @default.
- W1569365736 hasConceptScore W1569365736C73766848 @default.
- W1569365736 hasConceptScore W1569365736C86339819 @default.
- W1569365736 hasConceptScore W1569365736C86803240 @default.
- W1569365736 hasConceptScore W1569365736C89604277 @default.
- W1569365736 hasConceptScore W1569365736C98274493 @default.
- W1569365736 hasIssue "6" @default.
- W1569365736 hasLocation W15693657361 @default.
- W1569365736 hasLocation W15693657362 @default.
- W1569365736 hasOpenAccess W1569365736 @default.
- W1569365736 hasPrimaryLocation W15693657361 @default.
- W1569365736 hasRelatedWork W1569365736 @default.
- W1569365736 hasRelatedWork W1595670287 @default.
- W1569365736 hasRelatedWork W1972038116 @default.
- W1569365736 hasRelatedWork W1992935306 @default.
- W1569365736 hasRelatedWork W2024331178 @default.
- W1569365736 hasRelatedWork W2030552028 @default.
- W1569365736 hasRelatedWork W2073788452 @default.
- W1569365736 hasRelatedWork W2141856214 @default.
- W1569365736 hasRelatedWork W2157508941 @default.
- W1569365736 hasRelatedWork W2288526040 @default.
- W1569365736 hasVolume "146" @default.
- W1569365736 isParatext "false" @default.
- W1569365736 isRetracted "false" @default.
- W1569365736 magId "1569365736" @default.
- W1569365736 workType "article" @default.