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• W1569383111 abstract "The purpose of the present study is to examine the effects of essential oil of Citrus bergamia Risso (bergamot, BEO) on intracellular Ca 2+ in human umbilical vein endothelial cells. Fura-2 fluorescence was used to examine changes in intracellular Ca 2+ concentration<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M1><mml:mrow><mml:msub><mml:mrow><mml:mrow><mml:mo stretchy=false>[</mml:mo><mml:mrow><mml:msup><mml:mrow><mml:mtext>Ca</mml:mtext></mml:mrow><mml:mrow><mml:mn mathvariant=normal>2</mml:mn><mml:mo>+</mml:mo></mml:mrow></mml:msup></mml:mrow><mml:mo stretchy=false>]</mml:mo></mml:mrow></mml:mrow><mml:mrow><mml:mtext>i</mml:mtext></mml:mrow></mml:msub></mml:mrow></mml:math>. In the presence of extracellular Ca 2+ , BEO increased<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M2><mml:mrow><mml:msub><mml:mrow><mml:mrow><mml:mo stretchy=false>[</mml:mo><mml:mrow><mml:msup><mml:mrow><mml:mtext>Ca</mml:mtext></mml:mrow><mml:mrow><mml:mn mathvariant=normal>2</mml:mn><mml:mo>+</mml:mo></mml:mrow></mml:msup></mml:mrow><mml:mo stretchy=false>]</mml:mo></mml:mrow></mml:mrow><mml:mrow><mml:mtext>i</mml:mtext></mml:mrow></mml:msub></mml:mrow></mml:math>, which was partially inhibited by a nonselective Ca 2+ channel blocker La 3+ . In Ca 2+ -free extracellular solutions, BEO increased<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M3><mml:mrow><mml:msub><mml:mrow><mml:mrow><mml:mo stretchy=false>[</mml:mo><mml:mrow><mml:msup><mml:mrow><mml:mtext>Ca</mml:mtext></mml:mrow><mml:mrow><mml:mn mathvariant=normal>2</mml:mn><mml:mo>+</mml:mo></mml:mrow></mml:msup></mml:mrow><mml:mo stretchy=false>]</mml:mo></mml:mrow></mml:mrow><mml:mrow><mml:mtext>i</mml:mtext></mml:mrow></mml:msub></mml:mrow></mml:math>in a concentration-dependent manner, suggesting that BEO mobilizes intracellular Ca 2+ . BEO-induced<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M4><mml:mrow><mml:msub><mml:mrow><mml:mrow><mml:mo stretchy=false>[</mml:mo><mml:mrow><mml:msup><mml:mrow><mml:mtext>Ca</mml:mtext></mml:mrow><mml:mrow><mml:mn mathvariant=normal>2</mml:mn><mml:mo>+</mml:mo></mml:mrow></mml:msup></mml:mrow><mml:mo stretchy=false>]</mml:mo></mml:mrow></mml:mrow><mml:mrow><mml:mtext>i</mml:mtext></mml:mrow></mml:msub></mml:mrow></mml:math>increase was partially inhibited by a Ca 2+ -induced Ca 2+ release inhibitor dantrolene, a phospholipase C inhibitor U73122, and an inositol 1,4,5-triphosphate (IP 3 )-gated Ca 2+ channel blocker, 2-aminoethoxydiphenyl borane (2-APB). BEO also increased<mml:math xmlns:mml=http://www.w3.org/1998/Math/MathML id=M5><mml:mrow><mml:msub><mml:mrow><mml:mrow><mml:mo stretchy=false>[</mml:mo><mml:mrow><mml:msup><mml:mrow><mml:mtext>Ca</mml:mtext></mml:mrow><mml:mrow><mml:mn mathvariant=normal>2</mml:mn><mml:mo>+</mml:mo></mml:mrow></mml:msup></mml:mrow><mml:mo stretchy=false>]</mml:mo></mml:mrow></mml:mrow><mml:mrow><mml:mtext>i</mml:mtext></mml:mrow></mml:msub></mml:mrow></mml:math>in the presence of carbonyl cyanide m-chlorophenylhydrazone, an inhibitor of mitochondrial Ca 2+ uptake. In addition, store-operated Ca 2+ entry (SOC) was potentiated by BEO. These results suggest that BEO mobilizes Ca 2+ from primary intracellular stores via Ca 2+ -induced and IP3-mediated Ca 2+ release and affect promotion of Ca 2+ influx, likely via an SOC mechanism." @default.
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• W1569383111 title "<i>Citrus bergamia</i>Risso Elevates Intracellular Ca^<b>2+</b>in Human Vascular Endothelial Cells due to Release of Ca^<b>2+</b>from Primary Intracellular Stores" @default.
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• W1569383111 doi "https://doi.org/10.1155/2013/759615" @default.
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