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- W1569390034 endingPage "1313" @default.
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- W1569390034 abstract "When the filamentous, nitrogen-fixing cyanobacterium Anabaena variabilis ATCC 29413 was subjected to nitrogen starvation under aerobic conditions, a complex series of events was initiated which resulted in heterocyst formation and derepression of the ability to fix dinitrogen. Using DNA-RNA hybridization techniques, we monitored the expression of several genes during nitrogen starvation and correlated changes in the mRNA levels with changes in enzyme activity, protein levels, and morphology. Nitrogenase mRNA was first observed after about 8.5 h of nitrogen starvation, as was nitrogenase activity. Late proheterocysts were present at that time. The level of nitrogenase mRNA increased for 5 to 6 h and then leveled off. Phycocyanin and allophycocyanin mRNA levels decreased rapidly within 1 h of nitrogen starvation; the levels increased later, as nitrogen starvation was alleviated, first by protein breakdown and then by nitrogen fixation. The average half-life of A. variabilis mRNA was determined by pulse-labeling techniques to be 16 to 18 min. Hybridization analysis showed that cpc and apc mRNAs also had half-lives of 16 to 18 min; the half-lives were not significantly different under nitrogen starvation conditions. Our results support the idea that the changes induced by nitrogen starvation are primarily the result of transcriptional regulation." @default.
- W1569390034 created "2016-06-24" @default.
- W1569390034 creator A5054510354 @default.
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- W1569390034 date "1989-03-01" @default.
- W1569390034 modified "2023-09-26" @default.
- W1569390034 title "Changes in gene expression during nitrogen starvation in Anabaena variabilis ATCC 29413" @default.
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- W1569390034 doi "https://doi.org/10.1128/jb.171.3.1309-1313.1989" @default.
- W1569390034 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/209746" @default.
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