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- W1569425200 abstract "A xylanase, which produces exclusively xylobiose from oat spelt and birch xylans, was isolated from the culture medium of Aeromonas caviae ME-1. The enzyme (xylanase V) was purified by ammonium sulfate fractionation, hydrophobic interaction, and ion-exchange and gel filtration chromatographies. The homogeneity of the final preparation was demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and agarose gel electrofocusing. The molecular mass and isoelectric point of the xylanase were 46 kDa and 5.4, respectively. Xylanase V had a maximum activity at a pH of 6.8 and at a temperature between 30 and 37°C. It was relatively stable at a pH between 5.0 and 8.6 and a temperature between 25 and 37°C. When soluble birch xylan was used as the substrate, the enzyme had a K m and V max of 2 mg/ml and 182 μmol of xylose equivalent liberated · min -1 · mg of protein -1 , respectively. By the action of xylanase V on xylans (from oat spelt and birch), only one product corresponding to xylobiose was observed by thin-layer chromatography. The xylanase V putative product was confirmed to be xylobiose by acid and enzymatic hydrolyses. The xylanase had neither β-xylosidase, α- l -arabinofuranosidase, cellulase, nor β-1,3-xylanase activities. Xylotriose was the shortest substrate which the enzyme could attack. These findings suggest that xylanase V is a novel enzyme that cleaves a xylobiose unit from one of the ends of xylans, probably by an exomechanism." @default.
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- W1569425200 date "1994-02-01" @default.
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- W1569425200 title "Purification and Characterization of <i>Aeromonas caviae</i> ME-1 Xylanase V, Which Produces Exclusively Xylobiose from Xylan" @default.
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- W1569425200 doi "https://doi.org/10.1128/aem.60.2.531-535.1994" @default.
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