Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569448190> ?p ?o ?g. }
- W1569448190 endingPage "271" @default.
- W1569448190 startingPage "268" @default.
- W1569448190 abstract "Objective: The severity and spread of Chikungunya fever in absence of effective antiviral therapy presents a serious public health threat. The present investigation aims to generate soluble and purified viral structural proteins that can be utilized to facilitate generation of reagents for development of both diagnostic and therapeutic measures. Methods: Bacterial expression system was used for optimization of expression and solubilization of structural proteins of CHIKV (Capsid, 6K and envelope proteins 1-3 [E1, E2 and E3]) as fusions with large (GST) and small (His and Strep) tags on a single platform. Affinity chromatography was used for small scale purification of viral proteins. Result: The effect of different tags, inducer concentrations, temperatures and duration of induction on solubilization of proteins has been optimized and small scale purification of all the structural proteins has been attempted. Utility of these solubilized proteins has been shown by analyzing the interaction of E2 with all the structural proteins using pull down assay. Conclusion: Small scale purification of all five structural proteins and ectodomains of envelope proteins E1 and E2 has been standardized. The data and reagents generated can be utilized for large scale purification and studying CHIKV biology. Keywords: Chikungunya virus, Protein expression, Solubilization, Purification." @default.
- W1569448190 created "2016-06-24" @default.
- W1569448190 creator A5005879554 @default.
- W1569448190 creator A5031670887 @default.
- W1569448190 creator A5055951785 @default.
- W1569448190 creator A5056083085 @default.
- W1569448190 creator A5070814115 @default.
- W1569448190 creator A5086740780 @default.
- W1569448190 date "2014-11-01" @default.
- W1569448190 modified "2023-09-23" @default.
- W1569448190 title "SMALL SCALE EXPRESSION, SOLUBILIZATION AND CHARACTERIZATION OF CHIKUNGUNYA VIRUS STRUCTURAL PROTEINS" @default.
- W1569448190 cites W1498408566 @default.
- W1569448190 cites W1518668585 @default.
- W1569448190 cites W1534754726 @default.
- W1569448190 cites W1918498186 @default.
- W1569448190 cites W1963590629 @default.
- W1569448190 cites W1964339709 @default.
- W1569448190 cites W1973562680 @default.
- W1569448190 cites W1977296748 @default.
- W1569448190 cites W1983177482 @default.
- W1569448190 cites W1988336483 @default.
- W1569448190 cites W1994821653 @default.
- W1569448190 cites W2000766338 @default.
- W1569448190 cites W2005227150 @default.
- W1569448190 cites W2009825851 @default.
- W1569448190 cites W2027796856 @default.
- W1569448190 cites W2032457529 @default.
- W1569448190 cites W2033427538 @default.
- W1569448190 cites W2034377840 @default.
- W1569448190 cites W2067779211 @default.
- W1569448190 cites W2086515925 @default.
- W1569448190 cites W2087276236 @default.
- W1569448190 cites W2097318849 @default.
- W1569448190 cites W2101642792 @default.
- W1569448190 cites W2103818416 @default.
- W1569448190 cites W2105260527 @default.
- W1569448190 cites W2112222932 @default.
- W1569448190 cites W2122697351 @default.
- W1569448190 cites W2128008808 @default.
- W1569448190 cites W2131836981 @default.
- W1569448190 cites W2141918346 @default.
- W1569448190 cites W2151156458 @default.
- W1569448190 cites W2151282930 @default.
- W1569448190 cites W2156251923 @default.
- W1569448190 cites W2167005142 @default.
- W1569448190 cites W2170169496 @default.
- W1569448190 cites W2201790399 @default.
- W1569448190 cites W2520104765 @default.
- W1569448190 hasPublicationYear "2014" @default.
- W1569448190 type Work @default.
- W1569448190 sameAs 1569448190 @default.
- W1569448190 citedByCount "0" @default.
- W1569448190 crossrefType "journal-article" @default.
- W1569448190 hasAuthorship W1569448190A5005879554 @default.
- W1569448190 hasAuthorship W1569448190A5031670887 @default.
- W1569448190 hasAuthorship W1569448190A5055951785 @default.
- W1569448190 hasAuthorship W1569448190A5056083085 @default.
- W1569448190 hasAuthorship W1569448190A5070814115 @default.
- W1569448190 hasAuthorship W1569448190A5086740780 @default.
- W1569448190 hasConcept C159047783 @default.
- W1569448190 hasConcept C179247698 @default.
- W1569448190 hasConcept C181199279 @default.
- W1569448190 hasConcept C185592680 @default.
- W1569448190 hasConcept C202878990 @default.
- W1569448190 hasConcept C2522874641 @default.
- W1569448190 hasConcept C2781273456 @default.
- W1569448190 hasConcept C4863142 @default.
- W1569448190 hasConcept C55493867 @default.
- W1569448190 hasConcept C86803240 @default.
- W1569448190 hasConceptScore W1569448190C159047783 @default.
- W1569448190 hasConceptScore W1569448190C179247698 @default.
- W1569448190 hasConceptScore W1569448190C181199279 @default.
- W1569448190 hasConceptScore W1569448190C185592680 @default.
- W1569448190 hasConceptScore W1569448190C202878990 @default.
- W1569448190 hasConceptScore W1569448190C2522874641 @default.
- W1569448190 hasConceptScore W1569448190C2781273456 @default.
- W1569448190 hasConceptScore W1569448190C4863142 @default.
- W1569448190 hasConceptScore W1569448190C55493867 @default.
- W1569448190 hasConceptScore W1569448190C86803240 @default.
- W1569448190 hasIssue "5" @default.
- W1569448190 hasLocation W15694481901 @default.
- W1569448190 hasOpenAccess W1569448190 @default.
- W1569448190 hasPrimaryLocation W15694481901 @default.
- W1569448190 hasRelatedWork W1560851439 @default.
- W1569448190 hasRelatedWork W1607294761 @default.
- W1569448190 hasRelatedWork W188468884 @default.
- W1569448190 hasRelatedWork W1968019435 @default.
- W1569448190 hasRelatedWork W1971346164 @default.
- W1569448190 hasRelatedWork W1994901735 @default.
- W1569448190 hasRelatedWork W2056153539 @default.
- W1569448190 hasRelatedWork W2080171591 @default.
- W1569448190 hasRelatedWork W2093759905 @default.
- W1569448190 hasRelatedWork W2142114240 @default.
- W1569448190 hasRelatedWork W2168918818 @default.
- W1569448190 hasRelatedWork W2291208271 @default.
- W1569448190 hasRelatedWork W2316144419 @default.
- W1569448190 hasRelatedWork W2419372122 @default.
- W1569448190 hasRelatedWork W2558529783 @default.