FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569483952> ?p ?o ?g. }

• W1569483952 endingPage "401" @default.
• W1569483952 startingPage "395" @default.
• W1569483952 abstract "Human erythrocytes have a life span of about 120 days. One can easily separate young and aged cells by density centrifugation. For these reasons they are especially suited for studying ageing processes. There is general agreement that the activity of several glycolytic enzymes decreases during ageing. However, nothing is known about typical membrane enzymes in connection with ageing, and since it was felt that membrane alterations might be of primary importance, a comparative study of phospholipid-metabolizing enzymes was undertaken. The enzymes studied in this investigation were: a) the structure-bound acid: CoA ligase (AMP) and acyl-CoA: Iysolecithin-O-acyltransferase which together incorporate lysolecithin and fatty acid into membrane bound lecithin. b) lysophospholipase (lysolecithin acyl-hydrolase) which degrades lysolecithin into glycerophosphorylcholine plus fatty acid. Human erythrocytes from healthy donors were separated by simple ultracentrifugation and by albumin density centrifugation according to age. The mean volume of the young erythrocytes was 109 μ3, while that of the aged cells was 74 μ3. The enzyme activities of freeze-thawed cells were determined and corrected for cell numbers. Furthermore, the enzyme activities-in hemoglobin-free stroma were measured. [14C]-labelled substrates were used to determine the enzyme activities: [1-14C]oleic acid for the acylation of lysolecithin and [1-acyl-14C]lysolecithin for the lysophospholipase reaction. The results showed that the activities of both enzymic reactions as measured with old cells were 40 to 50% lower than the activities found in young cells. In hemolysates from young and old cells the following enzyme activities were determined: the acylating activity decreased from 117 to 67 MolX10−20/cell×min, and the lysophospholipase activity from 185 to 123 Mol×10−20/cellXmin. The ATP content of old cells was only slightly lower than that of young cells. Comparative analyses of erythrocytes stored in acid citric dextrose for two months at 4° gave different results: these cells which had been “aged in vitro” had an extremely low ATP content, while the activities of the lysolecithin metabolizing enzymes remained unchanged. These findings suggest that the lysolecithin metabolism has a significance in cell ageing processes. It remains to be shown, however, that lysolecithin produced by macrophages lining the RES sinuses is the decisive factor responsible for the death and removal of aged cells." @default.
• W1569483952 created "2016-06-24" @default.
• W1569483952 creator A5054216675 @default.
• W1569483952 creator A5067647059 @default.
• W1569483952 creator A5067671632 @default.
• W1569483952 creator A5068036526 @default.
• W1569483952 date "1968-08-01" @default.
• W1569483952 modified "2023-09-30" @default.
• W1569483952 title "Lysolecithin-Stoffwechsel in Erythrocytenmembranen. Lysolecithin-Acylierung und Lysophospholipase in alternden Erythrocyten" @default.
• W1569483952 cites W1977763680 @default.
• W1569483952 cites W1989446711 @default.
• W1569483952 cites W1997117449 @default.
• W1569483952 cites W2000305307 @default.
• W1569483952 cites W2011850160 @default.
• W1569483952 cites W2027404083 @default.
• W1569483952 cites W2035215843 @default.
• W1569483952 cites W2112162433 @default.
• W1569483952 cites W2270988517 @default.
• W1569483952 cites W2322045801 @default.
• W1569483952 cites W2397461281 @default.
• W1569483952 cites W4297683415 @default.
• W1569483952 doi "https://doi.org/10.1111/j.1432-1033.1968.tb00382.x" @default.
• W1569483952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/5680357" @default.
• W1569483952 hasPublicationYear "1968" @default.
• W1569483952 type Work @default.
• W1569483952 sameAs 1569483952 @default.
• W1569483952 citedByCount "20" @default.
• W1569483952 crossrefType "journal-article" @default.
• W1569483952 hasAuthorship W1569483952A5054216675 @default.
• W1569483952 hasAuthorship W1569483952A5067647059 @default.
• W1569483952 hasAuthorship W1569483952A5067671632 @default.
• W1569483952 hasAuthorship W1569483952A5068036526 @default.
• W1569483952 hasBestOaLocation W15694839521 @default.
• W1569483952 hasConcept C12927208 @default.
• W1569483952 hasConcept C181199279 @default.
• W1569483952 hasConcept C185592680 @default.
• W1569483952 hasConcept C2778530336 @default.
• W1569483952 hasConcept C2778918659 @default.
• W1569483952 hasConcept C2779564620 @default.
• W1569483952 hasConcept C2781403372 @default.
• W1569483952 hasConcept C28212737 @default.
• W1569483952 hasConcept C41625074 @default.
• W1569483952 hasConcept C43617362 @default.
• W1569483952 hasConcept C543025807 @default.
• W1569483952 hasConcept C55493867 @default.
• W1569483952 hasConcept C7623868 @default.
• W1569483952 hasConceptScore W1569483952C12927208 @default.
• W1569483952 hasConceptScore W1569483952C181199279 @default.
• W1569483952 hasConceptScore W1569483952C185592680 @default.
• W1569483952 hasConceptScore W1569483952C2778530336 @default.
• W1569483952 hasConceptScore W1569483952C2778918659 @default.
• W1569483952 hasConceptScore W1569483952C2779564620 @default.
• W1569483952 hasConceptScore W1569483952C2781403372 @default.
• W1569483952 hasConceptScore W1569483952C28212737 @default.
• W1569483952 hasConceptScore W1569483952C41625074 @default.
• W1569483952 hasConceptScore W1569483952C43617362 @default.
• W1569483952 hasConceptScore W1569483952C543025807 @default.
• W1569483952 hasConceptScore W1569483952C55493867 @default.
• W1569483952 hasConceptScore W1569483952C7623868 @default.
• W1569483952 hasIssue "3" @default.
• W1569483952 hasLocation W15694839521 @default.
• W1569483952 hasLocation W15694839522 @default.
• W1569483952 hasOpenAccess W1569483952 @default.
• W1569483952 hasPrimaryLocation W15694839521 @default.
• W1569483952 hasRelatedWork W1569483952 @default.
• W1569483952 hasRelatedWork W1964918725 @default.
• W1569483952 hasRelatedWork W1972665833 @default.
• W1569483952 hasRelatedWork W2045173278 @default.
• W1569483952 hasRelatedWork W2048465125 @default.
• W1569483952 hasRelatedWork W2076739248 @default.
• W1569483952 hasRelatedWork W2138772345 @default.
• W1569483952 hasRelatedWork W2149532295 @default.
• W1569483952 hasRelatedWork W4233500611 @default.
• W1569483952 hasRelatedWork W2141253507 @default.
• W1569483952 hasVolume "5" @default.
• W1569483952 isParatext "false" @default.
• W1569483952 isRetracted "false" @default.
• W1569483952 magId "1569483952" @default.
• W1569483952 workType "article" @default.