Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569503198> ?p ?o ?g. }
- W1569503198 endingPage "55" @default.
- W1569503198 startingPage "46" @default.
- W1569503198 abstract "A cytoplasmic peptide:N-glycanase (PNGase) has been implicated in the proteasomal degradation of aberrant glycoproteins synthesized in the endoplasmic reticulum. The reaction is believed to be important for subsequent proteolysis by the proteasome since bulky N-glycan chains on misfolded glycoproteins may impair their efficient entry into the interior of the cylinder-shaped 20S proteasome, where the active sites of the proteases reside. The deglycosylation reaction by PNGase brings about two major changes on substrate proteins; one is a removal of N-glycan chains, and the other is the introduction of negative charge(s) into the core peptide by converting glycosylated asparagine residue(s) into aspartic acid residue(s). Therefore, PNGase action can be accurately monitored by detecting both changes using two different methods; that is, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for deglycosylation and isoelectric focusing for detection of introduction of negative charge(s) into core proteins. This chapter will describe the simple in vivo as well as in vitro assay method to detect PNGase activity." @default.
- W1569503198 created "2016-06-24" @default.
- W1569503198 creator A5038752989 @default.
- W1569503198 creator A5054250942 @default.
- W1569503198 creator A5071234506 @default.
- W1569503198 date "2006-01-01" @default.
- W1569503198 modified "2023-09-26" @default.
- W1569503198 title "A Cytoplasmic Peptide: N‐Glycanase" @default.
- W1569503198 cites W1497028439 @default.
- W1569503198 cites W1515920608 @default.
- W1569503198 cites W1557901683 @default.
- W1569503198 cites W1915442041 @default.
- W1569503198 cites W1967219468 @default.
- W1569503198 cites W1971987201 @default.
- W1569503198 cites W1976601347 @default.
- W1569503198 cites W1981551685 @default.
- W1569503198 cites W1982944039 @default.
- W1569503198 cites W1983413528 @default.
- W1569503198 cites W1989400359 @default.
- W1569503198 cites W2005763639 @default.
- W1569503198 cites W2013921979 @default.
- W1569503198 cites W2014788821 @default.
- W1569503198 cites W2019310038 @default.
- W1569503198 cites W2031079874 @default.
- W1569503198 cites W2036406080 @default.
- W1569503198 cites W2046933512 @default.
- W1569503198 cites W2049656093 @default.
- W1569503198 cites W2052858784 @default.
- W1569503198 cites W2053021234 @default.
- W1569503198 cites W2055242244 @default.
- W1569503198 cites W2061663235 @default.
- W1569503198 cites W2065022563 @default.
- W1569503198 cites W2068116493 @default.
- W1569503198 cites W2071546385 @default.
- W1569503198 cites W2073769547 @default.
- W1569503198 cites W2077404304 @default.
- W1569503198 cites W2080404164 @default.
- W1569503198 cites W2081314925 @default.
- W1569503198 cites W2082788676 @default.
- W1569503198 cites W2085648931 @default.
- W1569503198 cites W2089844599 @default.
- W1569503198 cites W2103110518 @default.
- W1569503198 cites W2105435074 @default.
- W1569503198 cites W2106778710 @default.
- W1569503198 cites W2110525487 @default.
- W1569503198 cites W2113164415 @default.
- W1569503198 cites W2117313136 @default.
- W1569503198 cites W2126753022 @default.
- W1569503198 cites W2149761960 @default.
- W1569503198 cites W2151367783 @default.
- W1569503198 cites W2151608616 @default.
- W1569503198 cites W2160535967 @default.
- W1569503198 cites W2163412569 @default.
- W1569503198 doi "https://doi.org/10.1016/s0076-6879(06)15004-1" @default.
- W1569503198 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17116467" @default.
- W1569503198 hasPublicationYear "2006" @default.
- W1569503198 type Work @default.
- W1569503198 sameAs 1569503198 @default.
- W1569503198 citedByCount "30" @default.
- W1569503198 countsByYear W15695031982012 @default.
- W1569503198 countsByYear W15695031982013 @default.
- W1569503198 countsByYear W15695031982014 @default.
- W1569503198 countsByYear W15695031982015 @default.
- W1569503198 countsByYear W15695031982016 @default.
- W1569503198 countsByYear W15695031982019 @default.
- W1569503198 countsByYear W15695031982020 @default.
- W1569503198 countsByYear W15695031982021 @default.
- W1569503198 countsByYear W15695031982022 @default.
- W1569503198 crossrefType "book-chapter" @default.
- W1569503198 hasAuthorship W1569503198A5038752989 @default.
- W1569503198 hasAuthorship W1569503198A5054250942 @default.
- W1569503198 hasAuthorship W1569503198A5071234506 @default.
- W1569503198 hasConcept C108625454 @default.
- W1569503198 hasConcept C116084860 @default.
- W1569503198 hasConcept C139447449 @default.
- W1569503198 hasConcept C158617107 @default.
- W1569503198 hasConcept C158619295 @default.
- W1569503198 hasConcept C181199279 @default.
- W1569503198 hasConcept C185592680 @default.
- W1569503198 hasConcept C190062978 @default.
- W1569503198 hasConcept C206212055 @default.
- W1569503198 hasConcept C2775895851 @default.
- W1569503198 hasConcept C2776817266 @default.
- W1569503198 hasConcept C2777313579 @default.
- W1569503198 hasConcept C2779281246 @default.
- W1569503198 hasConcept C2781307694 @default.
- W1569503198 hasConcept C50476208 @default.
- W1569503198 hasConcept C515207424 @default.
- W1569503198 hasConcept C55493867 @default.
- W1569503198 hasConceptScore W1569503198C108625454 @default.
- W1569503198 hasConceptScore W1569503198C116084860 @default.
- W1569503198 hasConceptScore W1569503198C139447449 @default.
- W1569503198 hasConceptScore W1569503198C158617107 @default.
- W1569503198 hasConceptScore W1569503198C158619295 @default.
- W1569503198 hasConceptScore W1569503198C181199279 @default.
- W1569503198 hasConceptScore W1569503198C185592680 @default.
- W1569503198 hasConceptScore W1569503198C190062978 @default.
- W1569503198 hasConceptScore W1569503198C206212055 @default.