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• W1569515040 abstract "This chapter discusses that the Atomic force microscope (AFM) measurements can investigate aspects of bacteria‐surface interactions inaccessible to more traditional microbiology techniques, such as optical microscopy or batch culture experiments.. AFM is a scanning probe microscopy technique that provides information about a localized portion of a sample by sensing the behavior of a small tip‐cantilever system, which interacts with the sample. The chapter covers AFM force measurement by functionalizing the AFM probe that is done by colloid bead probe and bacteria probe or biologically active force probe method. Force-sensing cantilevers can be used to measure forces between a bacterium on a glass cover slip and silicon or silicon nitride tip. A bead coated with living cells is attached to the end of a cantilever to measure forces between two cells or between a bacterium and a specific face of a mineral. This chapter concludes by presenting two examples of AFM force measurements for a bacterium and material substrate in aqueous solution: forces detected as a bacterium approaches a material surface and forces detected as a bacterium is pulled away from a material surface. A great deal of care and perseverance are required to extract valid and meaningful conclusions from the force measurements. In spite of the difficulties involved, there is no doubt that AFM experiments will continue to enhance the understanding of the nature of bacteria‐surface interactions." @default.
• W1569515040 created "2016-06-24" @default.
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• W1569515040 date "2005-01-01" @default.
• W1569515040 modified "2023-10-14" @default.
• W1569515040 title "Force Measurements Between a Bacterium and Another Surface In Situ" @default.
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• W1569515040 doi "https://doi.org/10.1016/s0065-2164(05)58003-1" @default.
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