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• W1569560298 abstract "Pleiotropic mutations (xcp) affecting the secretion of proteins in Pseudomonas aeruginosa have been previously characterized and mapped at three different loci on the chromosome. The xcp-1 gene, which is located at the 0-min region, was isolated from a genomic bank containing DNA from P. aeruginosa PAO1. The recombinant cosmid pLX25 complemented the xcp::Tn5-751 insertion mutation previously described. The xcp-1 gene was located on pLX25 by mapping the insertion point of transposon Tn5-751 and by deletion and subcloning analysis. The xcp-1 gene was expressed when transcription was initiated from a tac promoter, and a 26,000-dalton protein was identified in Escherichia coli minicells. The Xcp-1 protein was associated with the membrane fraction of E. coli. A 30-kilodalton outer membrane protein was also affected by the xcp::Tn5-751 mutation in P. aeruginosa. The possible correlation between Xcp-1 and this protein is discussed." @default.
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• W1569560298 date "1989-08-01" @default.
• W1569560298 modified "2023-10-16" @default.
• W1569560298 title "Protein secretion in Pseudomonas aeruginosa: molecular cloning and characterization of the xcp-1 gene" @default.
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• W1569560298 doi "https://doi.org/10.1128/jb.171.8.4342-4348.1989" @default.
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