FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1569598281> ?p ?o ?g. }

• W1569598281 endingPage "3710" @default.
• W1569598281 startingPage "3703" @default.
• W1569598281 abstract "Abstract The role of GTP-binding proteins in autophagic vacuole formation was investigated in isolated rat hepatocytes permeabilized by alpha-toxin from Staphylococcus aureus, an agent which creates stable plasma membrane channels allowing exchange of small (< or = 1000 Da) molecules. Vacuole formation was monitored from the uptake of 125I-tyramine-cellobiitol (125ITC) into osmotically sensitive vacuoles isolated on colloidal silica density gradients. Separation was based on an established observation that autophagic vacuoles are retained in a heavy midgradient band when samples are layered, but are selectively shifted to dense fractions when they are previously dispersed in the gradient material. The vacuolar uptake of 125ITC was concentration-dependent and required exogenous ATP: 94% was directly mediated by sequestration; 6% was acquired by fluid-phase endocytosis as monitored by [carboxyl-14C]dextran-carboxyl. Although the amino acid control of proteolysis was lost, addition of the nonhydrolyzable GTP analog GTP gamma S (as well as GMP-PNP) decreased fractional rates of direct vacuolar 125ITC uptake and long-lived proteolysis by similar amounts (1.02-1.03% h-1), substantiating the notion that the effects were the direct result of autophagic inhibition. These and associated findings, supported by quantitative electron microscopy, indicate the presence of ongoing macro- and microautophagy in alpha-toxin-permeabilized cells and suggest that one or more GTP-binding proteins is required in macroautophagic vacuole formation." @default.
• W1569598281 created "2016-06-24" @default.
• W1569598281 creator A5008479616 @default.
• W1569598281 creator A5035311248 @default.
• W1569598281 creator A5052031158 @default.
• W1569598281 creator A5076278641 @default.
• W1569598281 date "1994-02-01" @default.
• W1569598281 modified "2023-10-15" @default.
• W1569598281 title "De novo autophagic vacuole formation in hepatocytes permeabilized by Staphylococcus aureus alpha-toxin. Inhibition by nonhydrolyzable GTP analogs." @default.
• W1569598281 cites W1480149069 @default.
• W1569598281 cites W1490154674 @default.
• W1569598281 cites W1515071563 @default.
• W1569598281 cites W1516800253 @default.
• W1569598281 cites W1529494662 @default.
• W1569598281 cites W1532284082 @default.
• W1569598281 cites W1572145162 @default.
• W1569598281 cites W1584945129 @default.
• W1569598281 cites W1593406565 @default.
• W1569598281 cites W1616155428 @default.
• W1569598281 cites W1823433937 @default.
• W1569598281 cites W1963510706 @default.
• W1569598281 cites W1985700603 @default.
• W1569598281 cites W1994495928 @default.
• W1569598281 cites W1996177900 @default.
• W1569598281 cites W2000622138 @default.
• W1569598281 cites W2014987538 @default.
• W1569598281 cites W2026876894 @default.
• W1569598281 cites W2028343006 @default.
• W1569598281 cites W2033122648 @default.
• W1569598281 cites W2035900036 @default.
• W1569598281 cites W2036958086 @default.
• W1569598281 cites W2042963074 @default.
• W1569598281 cites W2060215125 @default.
• W1569598281 cites W2061813775 @default.
• W1569598281 cites W2080695903 @default.
• W1569598281 cites W2089393609 @default.
• W1569598281 cites W2094235261 @default.
• W1569598281 cites W2094269336 @default.
• W1569598281 cites W2155330019 @default.
• W1569598281 cites W2158542693 @default.
• W1569598281 cites W2181273178 @default.
• W1569598281 cites W2287882780 @default.
• W1569598281 cites W2407381613 @default.
• W1569598281 cites W2415623248 @default.
• W1569598281 cites W2415966919 @default.
• W1569598281 cites W2431809059 @default.
• W1569598281 doi "https://doi.org/10.1016/s0021-9258(17)41917-x" @default.
• W1569598281 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8106415" @default.
• W1569598281 hasPublicationYear "1994" @default.
• W1569598281 type Work @default.
• W1569598281 sameAs 1569598281 @default.
• W1569598281 citedByCount "28" @default.
• W1569598281 countsByYear W15695982812014 @default.
• W1569598281 countsByYear W15695982812015 @default.
• W1569598281 crossrefType "journal-article" @default.
• W1569598281 hasAuthorship W1569598281A5008479616 @default.
• W1569598281 hasAuthorship W1569598281A5035311248 @default.
• W1569598281 hasAuthorship W1569598281A5052031158 @default.
• W1569598281 hasAuthorship W1569598281A5076278641 @default.
• W1569598281 hasBestOaLocation W15695982811 @default.
• W1569598281 hasConcept C102568950 @default.
• W1569598281 hasConcept C118716 @default.
• W1569598281 hasConcept C159110408 @default.
• W1569598281 hasConcept C181199279 @default.
• W1569598281 hasConcept C185592680 @default.
• W1569598281 hasConcept C190062978 @default.
• W1569598281 hasConcept C190283241 @default.
• W1569598281 hasConcept C203522944 @default.
• W1569598281 hasConcept C2775944032 @default.
• W1569598281 hasConcept C2777367657 @default.
• W1569598281 hasConcept C2779489039 @default.
• W1569598281 hasConcept C49453240 @default.
• W1569598281 hasConcept C523546767 @default.
• W1569598281 hasConcept C54355233 @default.
• W1569598281 hasConcept C55493867 @default.
• W1569598281 hasConcept C64943373 @default.
• W1569598281 hasConcept C71924100 @default.
• W1569598281 hasConcept C86803240 @default.
• W1569598281 hasConceptScore W1569598281C102568950 @default.
• W1569598281 hasConceptScore W1569598281C118716 @default.
• W1569598281 hasConceptScore W1569598281C159110408 @default.
• W1569598281 hasConceptScore W1569598281C181199279 @default.
• W1569598281 hasConceptScore W1569598281C185592680 @default.
• W1569598281 hasConceptScore W1569598281C190062978 @default.
• W1569598281 hasConceptScore W1569598281C190283241 @default.
• W1569598281 hasConceptScore W1569598281C203522944 @default.
• W1569598281 hasConceptScore W1569598281C2775944032 @default.
• W1569598281 hasConceptScore W1569598281C2777367657 @default.
• W1569598281 hasConceptScore W1569598281C2779489039 @default.
• W1569598281 hasConceptScore W1569598281C49453240 @default.
• W1569598281 hasConceptScore W1569598281C523546767 @default.
• W1569598281 hasConceptScore W1569598281C54355233 @default.
• W1569598281 hasConceptScore W1569598281C55493867 @default.
• W1569598281 hasConceptScore W1569598281C64943373 @default.
• W1569598281 hasConceptScore W1569598281C71924100 @default.
• W1569598281 hasConceptScore W1569598281C86803240 @default.
• W1569598281 hasIssue "5" @default.
• W1569598281 hasLocation W15695982811 @default.

• next