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- W1569678841 abstract "This chapter discusses protein enrichment approaches prior to the analysis, particularly the proteomic analysis to the use of two-dimensional (2D) electrophoresis. The proteomic analysis of bacterial proteins detects mainly the high-abundance, hydrophilic components of a mixture. For the detection of the low abundance proteins, a separation of the complex mixture into fractions with fewer components is necessary. This can be achieved using biochemical approaches, such as centrifugation and chromatography. A combination of separation techniques might be more efficient. Even if a protein has been sufficiently enriched, it is possible that it cannot be detected because of limitations in the separation and visualization methods, mainly in 2D electrophoresis. Moreover, the analysis of the proteomes of higher organisms is anticipated to be more complex in comparison with the bacterial proteomes and may probably require a combination of many analytical approaches, comprising multiple enrichment steps and efficient separation and detection methods." @default.
- W1569678841 created "2016-06-24" @default.
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- W1569678841 date "2002-01-01" @default.
- W1569678841 modified "2023-10-18" @default.
- W1569678841 title "Enrichment and proteomic analysis of low-abundance bacterial proteins" @default.
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- W1569678841 doi "https://doi.org/10.1016/s0076-6879(02)58096-4" @default.
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